開發多功能生物球果蠅測試平台

Development of multi-function bio-sphere Drosophila test platform

梁浩瑜(Liang, Hao Yu)

指導教授 ： 方維倫

國立清華大學/工學院/動力機械工程學系/碩士(2015年)

摘要

人類的大腦是非常精巧和複雜的器官，科學家們為了瞭解大腦的運作，會以簡單的果蠅大腦來進行研究，透過觀察果蠅的行為，加上生物技術之輔助來了解大腦的運作。但現今果蠅的行為實驗平台均為一維或二維的自由度，會造成侷限果蠅的行為，影響分析結果，並且大量的實驗也會耗費大量的人力，因此提出生物球果蠅實驗平台，將實驗所需的果蠅懲罰器與果蠅的學習標的，透過微機電技術和製程整合，製作成透明可撓之多功能薄膜，此薄膜再透過組裝貼附於壓克力透明球內，形成三維的果蠅測試環境，可使果蠅表現更完整的行為，而透明的結構可整合外部影像擷取系統自動化紀錄果蠅之行為，降低人力的消耗。
實驗平台之多功能薄膜為多層膜之結構，其整合電擊和熱刺激式做為果蠅之懲罰，藍光和琥珀光之發光二極體做為果蠅之學習標的，而多層膜之電性走線透過垂直導線，形成具有三維電性連結架構，來完成複雜的走線與電性牽引。未來還可以透過增加薄膜之層數來整合更多刺激與感測元件，使實驗平台之功能更加廣泛、完整。

關鍵字

生物球 ； 電擊式懲罰器 ； 熱刺激式懲罰器 ； 垂直導線 ； 撓性元件

並列摘要

Drosophila is a well-known vehicle for researchers to investigate the brain diseases, such as Alzheimer's disease and autism. Currently, due to the sensing technologies, most of the existing experiments only allow flies moving in single-dimension (tube) or two-dimension (arena). However, the flies have the native behavior of moving in 3D space. Therefore, this study establishes a bio-sphere Drosophila test platform to realize a natural like testing environment.
This study presents a novel “multi-function bio-sphere Drosophila test platform” implemented by Micro Electro Mechanical System (MEMS) and flexible electronics technology. The bio-sphere is consisted of a transparent rigid acrylic sphere covered with a transparent flexible MEMS bio-film with embedded micro transducers. The transparent bio-film can sense fly’s position, and stimulate flies by giving electric shock and heat. The multi-function bio-sphere test platform provides a natural like testing environment (sphere space) for fly’s behavior observation, and the future potential of automatic training and recording.

並列關鍵字

Bio-sphere ； Electrical punisher ； Heating punisher ； THV ； Flexible

參考文獻

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