以聚乳酸-甘醇酸及明膠作為高分子/陶瓷複合式支架軟骨層的研究

A study of Poly-DL lactide -co-glycolide acid and gelatin as the cartilage layer of polymer/ceramic composite scaffold

曾俊棋(Chun-Chi Tseng)

指導教授 ： 黃大仁

國立清華大學/工學院/化學工程學系/碩士(2009年)

摘要

本研究應用組織工程的概念，製得模擬軟硬骨結構的高分子/陶瓷複合式支架。此支架結構包含了軟骨層、分隔層及硬骨層。本研究以聚乳酸-甘醇酸(Poly-DL lactide -co-glycolide acid，PLGA)和明膠(Gelatin)這兩種高分子做為軟骨層，以三鈣磷酸鹽(tricalcium phosphate，TCP)作為分隔層，以聚磷酸鈣(calcium polyphosphate，CPP)作為硬骨層，並測試軟骨層與分隔層之間的連結力。
聚乳酸-甘醇酸與明膠皆為良好的生醫材料，我們將其製成多孔性支架並進行了體外細胞培養、機械性質分析及連結力測試以評估何種高分子適合作為此複合支架的軟骨層。
本研究結果發現明膠支架在細胞培養實驗及機械性質方面皆優於聚乳酸-甘醇酸，然而明膠與分隔層之間的連結力較弱。我們嘗試在軟骨層與分隔層之間燒結一層厚度為1mm的聚磷酸鈣作為連結層以增加軟骨層與分隔層間的連結力，結果發現明膠與分隔層之間的連結力有明顯的上升，而聚乳酸-甘醇酸與分隔層之間的連結力略微下降。此外，改變分隔層的燒結溫度，亦會對軟骨層與分隔層間的連結力有所影響。

關鍵字

組織工程 ； 軟骨 ； 明膠 ； 聚乳酸-甘醇酸 ； 複合支架

並列摘要

In this study,we used the concept of tissue engineering to fabricate the polymer/ceramic composite scaffold to imitate the articular joint. The structure of the scaffold includes cartilage layer,separation layer,and bone layer.We used Poly-DL lactide -co-glycolide acid(PLGA) and gelatin as the cartilage layer material,tricalcium phosphate(TCP) as the the separation layer material,and calcium polyphosphate(CPP) as the bone layer material.And the joining strength between the cartilage layer and separation layer were measured.
Poly-DL lactide -co-glycolide acid (PLGA) and gelatin are good biomaterials. We used these materials to produce porous scaffolds and these scaffolds were tested by in vitro cell culture,analysis of mechanical properties and joining strength test to assess which material of polymer is suitable for being the cartilage layer.
The results of the study conclude that the gelatin scaffold in the cell culture and mechanical properties are better than the PLGA scaffold,but the joining strength between the gelatin layer and separation layer is weaker.We tried to calcine a thickness of 1mm CPP between the cartilage layer and separation layer as the connecting layer to increase the joining strength. It’s found that the joining strength between the gelatin and separation layer increased evidently,but the the joining strength between the PLGA and separation layer decreased slightly. In addition,the change of the sintering temperature of the separation layer also made a great impact on the joining strength between cartilage layer and separation layer.

並列關鍵字

tissue engineering ； Cartilage ； Gelatin ； PLGA ； Composite scaffold

參考文獻

30. 簡千翔，明膠與陶瓷複合式支架培養關節軟骨細胞的研究，

1.W. Hunter, The structure and diseases of articulating cartilages, Philos Trans R Soc Lond 42 (1743) 514-521.

2. G. Shi, Q. Cai, C.Y. Wang, N. Lu, S. Wang, J. Bei, Fabrication and biocompatibility of cell scaffolds of poly(L-lactic acid) and poly-

5. J.A. Buckwalter, H.J. Mankin, Articular cartilage: tissue design and

6. 蔡傑次，明膠支架孔洞大小及培養條件對於組織工程軟骨形成的 影響，碩士論文，清華大學化工系(2008)。