介電泳細胞操控之生醫系統晶片設計與分析

Design and analysis of bio system chip via 3-D dielectrophoresis control

蘇宜貞(Yi-Chen Su)

指導教授 ： 劉承賢

國立清華大學/工學院/微機電工程研究所/碩士(2006年)

摘要

隨著科技的發展趨勢與知識的演進，許多領域的研究都逐漸對微小尺度下的現象及應用產生興趣，在生醫範疇的研究上尤其如此。生醫上許多新穎的研究題材，如光鉗或微鑷夾等，都是為了達到對細胞進行良好操縱之目的。由於目前研究的尺度不同於往，所以急需與細胞尺寸相符的新工具，許多的新方法與應用因應而生。在目前積極發展基因工程及單一細胞等相關研究的狀況下，微機電技術微小化的特色使其在生醫科技方面的應用與發展成為重要研究議題之一。本論文研究利用“介電泳”操控所設計的生醫系統晶片，可在微尺度下以非接觸力進行細胞操控，並可避免操控過程中對細胞產生傷害。
本論文以三維電場進行對細胞的操控，因此可操控的細胞分佈之高度範圍較大，有助於突破以往平面電極在有效工作高度上的限制。研究中利用平面電極與ITO玻璃建立三維電場，以省去上下電極對位之步驟，簡化製程難度。本文首先就過去相關的文獻進行探討，並以介電泳理論及CFD-RC軟體之數值模擬進行分析與驗證，進而依理論分析結果提出一整合性之生醫系統晶片，最後進行細胞實驗驗證此生醫系統晶片之可行性與功能。

關鍵字

介電泳

並列摘要

Along with the trend on technology development and the knowledge evolution, here gradually comes lots of interest on how the minute objects act and how we can really apply those objects into use in many research territories, especially in biomedicine category. There are so many new research topics in biotech category, such as the optical tweezers and micro-gripper etc. They are targeted for good manipulation of cell. Since the size of what people study differs from that of traditional studies, we need the tools which have the dimension compatible with the cell size. Therefore, it comes out many new methods and applications. Recently, under the condition of developing the related researches, such as genetic engineering and single cell…etc. actively, The special feature of Micro-Electrical-Mechanical-System (MEMS), miniaturization, makes itself become one of the hot research subjects in the biomedicine technology category. This bio-chip, which is presented in this thesis and designed based on the theory of dielectrophoresis（DEP）, can function for the manipulation of cells with non-contact force in micro scale. The non-contact force has the advantage of minimizing damage possibility.
The cell manipulation via three-dimensional electric fields in this thesis allows the more precise manipulation on cells and overcomes the drawback of cell manipulation via planed electrodes. This thesis first introduces the survey of literatures, propose a novel chip design and prove the feasibility of the design concepts via the theory of DEP and the numerical analysis with CFD-RC. Furthermore, we synthesize this bio-chip system on the basis of our numerical analysis. Finally, the feasibility and the functions of this bio-chip system are verified via bio experiments.

並列關鍵字

dielectrophoresis

參考文獻

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"Electro-Optical Platform for the Manipulation of Live Cells, " Langmuir 2003, 19, 1532-1538

[9] A. P. Lee, D. R. Ciarlo, P. A. Krulevitch, S. Lehew, J. Trevino, M. A. Northrup, "A Practical Microgripper by Fine Alignment, Eutectic Bonding and SMA Actuation," Tech. Dig., Transducers '95, 368-371, June 1995.