介電泳生物晶片應用於細胞裂解研究

The Application of Dielectrophoresis Biochip on Cell Lysis

余文程(Wen-cheng Yu)

指導教授 ： 魏哲弘

大同大學/工程學院/機械工程學系所/碩士(2006年)

摘要

本論文主要是研究將電極式的微晶片應用於細胞的裂解（lysis）與操控上。此技術相較於既往的大型機台的電穿孔技術而言，有許多的好處，其不僅可以增快裂解的效率、較容易擷取出細胞內物質做分析且其製造成本低廉，另外，對於無電極式晶片用於細胞的操作也有初步的研究。本研究計畫以康寧1737的玻璃為底材，在其上做微影蝕刻，分別蝕刻出電極式尖端型與無電極式漏斗型圖樣，其電極晶片電極間距約為50 m，流道約為250 m；無電極式則其流道入口寬度與壓縮尖端口比為500左右，而流道深度大約為30 m。實驗中所採用的細胞為酵母細胞（yeast）與人類的白血球細胞，電流源為直流脈衝的方式（亦有採用交流電源控制介電泳動），藉由控制電壓與頻率去操控與裂解細胞。同時將採用商用軟體Femlab與CFDRC做為電場的模擬分析，可瞭解電場分佈情形，進而與實驗結果比較。此技術將可以結合其他功能如PCR等成為一完整晶片，對於實驗室晶片（lab-on-a-chip）的研究，將具有莫大的助益。

關鍵字

裂解 ； 酵母細胞 ； 白血球 ； 實驗室晶片

並列摘要

This thesis studies cell lysis and dielectrophoresis cell manipulation with biochip. Compared to conventional technology, the MEMS based biochip technique has the advantages like the better efficiency, lower assay consumption, less power and easy manipulation. For cell lysis, the biochip was fabrication by using Corning 1737 as the substrate and the electrode was deposited by evaporation and patterned by lithography. The microchannels were made on PDMS by soft lithography. Two cells (yeast cell and human white cell) were used to for cell lysis. The minimum cell lysis operation condition is investigated by numerical analysis with commercial software Femlab. The simulation results agree with the experimental results very well. Different operation parameters like electrode size, applied voltage were applied to study the efficiency. The results show that when the size of the electrode increases, the efficiency of cell lysis increases. On the other hand, the cell lysis efficiency is very sensitive to the magnitude of the applied voltage and the length of the impulse. For lower applied voltage, the longer impulse will cause cell lysis while higher applied voltage, the impulse duration can be shorter. For electrodeless dielectrophoresis biochip, the yeast cell moves along the direction of the electric field. The speed of the cell movement increases with increasing applied voltage. The nature of the cell movement needs further investigation.

並列關鍵字

lysis ； yeast cells ； human white cell ； lab-on-a-chip

參考文獻

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