In recent years, along with display technology’s unceasing progress, more and more 3D displays entered into human life. The 3D display was considered as recent trends of development. Various manufacturers also respectively investigate and develop many stereo technologies in 3D displays, including stereoscoptic and autostereoscopic, and each classification has different stereoscopic vision production technology. Although every year new 3D displays appear in different ways, very few studies compared the effects of different types of 3D displays. Therefore, the purpose of this study is to establish a standard on different type stereo display in terms of ergonomics evaluations. In this research, we evaluated visual fatigue on three types of stereo displays and in different viewing time by experiment. The evaluation items of visual fatigue included accommodation time, CFF, and subjective fatigue. In addition, the stereo effects were also evaluated on different type of stereo displays, consisting of visual acuity, crosstalk level, and subjective evaluation on spatial, stereoscopic, and presence. The results revealed that different type of stereo displays caused different level of visual fatigue, and produced different levels of stereo effect. Visual fatigue was also affected by longer viewing time. Different type of displays could be recommended in different applications by the evaluation in this study.