製備腺嘌呤/金包覆之超順磁性奈米粒子於治療診斷之應用

Fabrication of Superparamagnetic Nanoparticles@adenine/ Au Hybrid Nanoparticles by Coordinated Polymerization in Theranostic Application

魏子強(Zi-Qiang Wei)

指導教授 ： 黃郁棻

國立清華大學/原子科學院/生醫工程與環境科學系/碩士(2014年)

https://doi.org/10.6843/NTHU.2014.00433

摘要

核甘酸與金屬離子可以藉由配位聚合的方式進行自組裝而製備出球狀膠體奈米粒子。此奈米粒子可透過包覆藥物分子及奈米尺度的載體提供多樣性的應用。在本研究中，將三磷酸腺苷修飾的超順磁性奈米粒子做為核並透過腺嘌呤與金離子以自組裝的方式在其外圍形成一不具晶形的保護層。自組裝的奈米粒子大小可經由反應時間、腺嘌呤濃度及金離子濃度之間的比例來完成。當腺嘌呤與金離子濃度比例為 1: 4時，經由穿透式電子顯微鏡鑑定結果其腺嘌呤/金奈米粒子之粒徑大小為140 nm 至 220 nm。此外，藉由載體自組裝過程中添加藥物或是染劑分子成功包覆於其中。本研究利用此新型自組裝藥物載體可應用於藥物傳輸系統，於細胞株 Tramp-C1 達到良好的治療效果。另外，研究中發現當加入1 mM 穀胱甘肽 (Glutathione)，發現酵素能有效的破壞載體結構而促使包覆內的藥物釋出，此具備控制與觸發應答性載體之特性於多項生醫領域中，具有良好且多功能的潛在前瞻性。

關鍵字

超分子 ； 自組裝 ； 腺嘌呤 ； 穀胱甘肽

並列摘要

Nucleotides and metal ions could assemble spontaneously in water to form spherical colloidal particles through coordination polymerization. The encapsulation of guest molecules and nanoscale materials in these supramolecular networks holds promise to tailor their functions for various applications. In this work, adenosine 5’-triphosphate modified porous hollow superparamagnetic iron oxide nanoparticles (PHNPs) was served as a scaffold for defining assembly of the amorphous shell from adenine and gold ions. The size of the hybrid nanoparticles could be controlled by adjusting reaction conditions such as reaction time, concentration, and the ratio of reactants. Transmission electron microscopy results revealed the formation of hierarchical self-assembled nanoparticles in sizes ranging from 140 nm to 220 nm in diameter. Moreover, fluorescent dyes and drug molecules were also encapsulated successfully during the self-assembly process. When mixing with glutathione, an observable increase of florescence indicated the gradual release of guest molecules from the dye-incorporated hybrid nanoparticles. Triggered release was also accessible to high-frequency magnetic field change. Overall, the present hybrid nanoparticles provide versatile functionalities in a variety of applications. Their utilization in drug delivery and controlled release is currently in progress.

並列關鍵字

supramolecular ； self-assembling ； adenine ； Glutathione

參考文獻

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