以奈米線指叉電極提升免疫分析晶片之偵測能力

本研究提出了結合銅指叉電極與鎳鈷合金奈米線之免疫分析晶片，利用了固定化金屬親和層析法 (Immobilized metal affinity chromatography,IMAC)，鎳鈷合金可與組胺酸標記蛋白質產生特異性結合之機制，在奈米線表面進行免疫反應。其中指叉電極是以黃光微影製程製作；鎳鈷合金奈米線則使用陽極氧化鋁模板，透過電化學的方式於孔洞中沉積鎳鈷合金，再經酸蝕刻的方式去除模板取得。實驗結果顯示，鎳鈷奈米線對His-tagged biotin 有特異性吸附能力且具有較大的表面積-體積比，因此相較於鎳鈷薄膜能夠吸附較多His-tagged biotin；而透過與指叉電極結合之感測器進行吸附能力檢測，可得到電流-電壓曲線並換算出響應值變化，其最低可量測到的濃度為142.9 ng/ml。另外利用此感測器進行免疫反應量測，得到之響應值與稀釋濃度的關係呈現負相關，且可測得之抗體最低濃度為714.2 ng/ml；而以螢光檢測免疫反應時，當抗體濃度到達1000 ng/ml 就無法量測出螢光值，此結果則確認出電性量測法擁有比螢光掃描法優異的靈敏度。

關鍵字

模板法；鎳鈷奈米線；組胺酸

並列摘要

This thesis presents an immunoassay analysis chip consisting of copper interdigitated electrodes (Cu-IDE) and nickel-cobalt alloy nanowires (Ni-Co NWs). The Ni-Co NWs have specific binding affinity with histidine-tagged proteins based on the principle of immobilized metal affinity chromatography (IMAC) so that the immunoassay can be performed on the Ni-Co NWs. The Cu-IDE was fabricated by the photolithography process; while the Ni-Co nanowires were synthesized by potentiostatic electrodeposition method using a porous anodic aluminum oxide (AAO) as template for nanowire growth. The experimental results show that Ni-Co NWs can immobilize more histidine-tagged biotins than Ni-Co film due to larger surface-to-volume ratio. The amount of the immobilized histidine-tagged biotin can be measured by means of current-voltage measurement if the concentration is greater than 142.9 ng/ml. After the immunoassays, the electrical response was found to be inversely proportioned to the concentration of the streptavidin-Cy5. The measurement limit was 714.2 ng/ml. However, the fluorescence intensity cannot be detected if the concentration of the streptavidin-Cy5 is lower than 1000 ng/ml. This proposed chip provides more sensitive electrical measurement than the fluorescent detection.

並列關鍵字

Template ； Ni-Co nanowires ； Histidine

參考文獻

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國際替代計量

以奈米線指叉電極提升免疫分析晶片之偵測能力

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