摘要
奈米線場效電晶體做為生物感測器，不僅擁有高靈敏度之優點外，亦兼具專一選擇性、即時回應、無標記檢測、極少量樣品需求，以及快速篩選的優異功能。在本文中，我們將介紹如何利用奈米線場效電晶體來偵測抗原與抗體的結合，同時亦可即時地監控單一活細胞受刺激時所臨場釋出之生化分子。此外，我們也設計了可重複使用的奈米線場效電晶體，做為可快速且高通量檢測蛋白質間交互作用的感測器。從實驗中得知鈣調素與心肌肌鈣蛋白結合時，至少需1μM的鈣離子來活化其作用。運用奈米線場效電晶體我們也證明了鈣調素與鈣離子通道有直接的交互作用。
並列摘要
Nanowire field-effect transistors have attracted great attention because they are an ideal biosensor with high sensitivity and selectivity, real-time response, and allows for label-free detection. In this article, we describe the uses of nanowire field-effect transistors to detect antigen-antibody associations and to monitor in situ the release of biochemical molecules from living cells. We have also developed a reusable nanowire field-effect transistor for rapid highthroughput screening protein interactions. In practice, we applied a calmodulin-modified nanowire transistor to study the troponin I-calmodulin interaction, in which the minimum concentration of Ca^(2+) required to activate calmodulin was determined to be 1 μM. We have also successfully demonstrated that the N-type Ca^(2+) channels expressed by cultured 293T cells can be recognized specifically by the calmodulin-modified nanowire transistor.
延伸閱讀
- 陳旻政、林家毅、林昌賢（2011）。奈米線場效電晶體平台製作及生物分子感測。國家奈米元件實驗室奈米通訊，18(4)，37-43。https://doi.org/10.29913/NDLC.201112.0007
- 林昕篁（2015）。矽奈米線場效電晶體應用於多種生物分子之感測〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2015.02105
- Hwang, R. Z. (2006). 奈米力學建構之感測器應用於生物分子辨識之研究 [doctoral dissertation, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2006.02626
- Hsiao, C. Y. (2010). 多晶矽奈米線場效電晶體於生物感測上的應用 [doctoral dissertation, National Chiao Tung University]. Airiti Library. https://doi.org/10.6842/NCTU.2010.00821
- 李彥霆（2010）。Silicon Nanowire Field Effect Transistor〔碩士論文，國立臺灣師範大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0021-1610201315202588