溫感性奈米碳管複合水膠於磁性與近紅外光加熱效用之研究

癌症始終名列主要死因之一，因此全世界皆積極發展新的治療技術，以降低其死亡率。磁性熱療(magnetic hyperthermia)與近紅外光熱治療 (photothermal therapy)，由於具高可行性與低副作用，已被視為最具潛力的癌症治療技術。有鑑於此，本實驗以溫感性水膠，搭載磁性氧化鐵/奈米碳管複合材料，發展出可以注射方式，對癌症組織進行磁性熱療與光熱治療的功能性複合水膠。本實驗成功利用水熱法合成出磁性氧化鐵奈米粒子(Fe3O4)，並利用界面活性劑poly(dimethyldiallylammonium chloride)(PDDA)，控制磁性氧化鐵奈米粒子的大小，亦成功表面改質奈米碳管，增強其於具生物相容性的poly (ethyleneoxide) -poly- (propylene oxide) –poly (ethylene oxide) (PEO-PPO-PEO)水膠的分散性。藉此製備出磁性氧化鐵(Fe3O4)/奈米碳管溫感性複合水膠。其為在室溫為溶膠態，體溫為凝膠態之可注射型複合水膠。並藉由外加磁場或近紅外光能量，誘發磁性氧化鐵、奈米碳管或是磁性奈米碳管釋出熱量，而升高複合水膠溫度，藉以殺死癌症細胞。本實驗發展的可注射溫感型複合水膠具多項創新優勢: 1.具磁場與近紅外光雙重熱治療功能:在外加磁場加熱下，上升溫度皆可達癌細胞凋亡溫度43℃;且在近紅外光照射下，加入越多混摻物，其複合水膠上升溫度越高。 2. 具可注射性，在室溫(25℃)為溶膠態，在體溫(37.5℃)為凝膠態。 3. 可藉由調整磁性奈米碳管的添加量，調控其相轉移溫度及加熱效果。此些優勢為提升癌症治療效果之關鍵，預期將開創多功能生醫材料新的研究里程碑，也為未來癌症治療方式提供更多選擇。

關鍵字

溫感性水膠；磁性奈米碳管；癌症熱療法

並列摘要

Cancer has been ranked one of the main causes of death, and therefore researchers are actively developing new treatment technologies to reduce its mortality. Magnetic hyperthermia and near-infrared photothermal therapy, due to their low side effects and high feasibility, have been considered as the most promising cancer therapies. Thus, the goal of this study is to utilize thermosensitive hydrogels as a carrier to deliver magnetic particle/ carbon nanotube (CNT) composites directly into cancerous tissue by injection. The injectable magnetic particle/ CNT composite hydrogels have the ability to treat cancers by both of magnetic hyperthermia and near-infrared photothermal therapy. This study successfully synthesized magnetic iron oxide nanoparticles, and also utilized poly(dimethyldiallylammonium chloride) surfactant to control the size of magnetic iron oxide nanoparticles. In addition, the surface of CNT was modified to improve their dispersibility in poly (ethyleneoxide) -poly- (propylene oxide) –poly (ethylene oxide) (PEO-PPO-PEO) hydrogel. The developed magnetic CNT composite hydrogel is injectable, which it is in sol state at room temperature and becomes gel state at body temperature. The temperature of the magnetic CNT composite hydrogel increased as applying alternating magnetic field or near- near-infrared irradiation to induce magnetic iron oxide nanoparticles or CNTs to release heat. The developed magnetic composite hydrogel have following advantages: 1.Bi-functional hydrogels for magnetic hyperthermia and near-infrared photothermal therapy. Experimental results show that temperature can increase to 43℃, which is the apoptosis temperature of cancer cells, by using an alternating magnetic field. Besides, more magnetic CNTs incorporated in hydrogels led to higher temperature of composite hydrogels during alternating magnetic field and near-infrared irradiation test. 2.Composite hydrogels with injectability. They are in sol-state at 25℃ while becomes gel-state at 37.5℃. 3.The sol-gel transition temperature and heating efficiency of the resulting composite hydrogels could be tuned by changing the incorporated amount of magnetic CNTs. These key advantages are critical and provide more options for treating cancers in the future. In addition, the results in this study are also important for developing multifunctional materials in bio-medical research.

並列關鍵字

injectable hydrogels；magnetic hyperthermia；near-infrared photothermal therapy

參考文獻

[ 34 ] 廖阿全,"PEG/PLGA/PEG水膠高分子的製備與其藥物釋放的研究,"國立台灣大學醫學工程研究所, 碩士論文 2004.

[ 53 ] 詹宗桂，鍾宜璋"磁場誘導藥物控制釋放之載體設計," 化學，2010,六十八卷,第三期,1-11 頁。

[ 78 ] 郭緒東, 陳奕澄, 張天長, "光療法在皮膚醫學上的概況與進展," 台灣醫界, 2011, 54, 3.

[ 1 ] J. Ferlay, I. Soe, R. Dikshit, S. Eser, C. Mathers, M. Rebelo, D.M. Parkin, D. Forman, F. Bray, "Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012," Int J Cancer. 2014, 136, E359

[ 2 ] J. A. Barreto, W. O’Malley, M. Kubeil, B. Graham, H. Stephan, L. Spiccia, "Nanomaterials: Applications in Cancer Imaging and Therapy," Adv. Mater. 2011, 23, H18

國際替代計量

溫感性奈米碳管複合水膠於磁性與近紅外光加熱效用之研究

全文下載

主題瀏覽