高價金屬塗料的抗菌效能和生物毒性研究

Antibacterial efficacy and biological evaluation of high-valence silver material

馬白彥(Brian S. Manurung)

指導教授 ： 陳怡寧

中原大學/理學院/生物科技研究所/碩士(2016年)

https://doi.org/10.6840/cycu201600540

摘要

藉由銀（Ag）塗料聚合物可提高產品抗菌功效。本研究計畫用以下四個設計塗料。第一，使用銀作為塗料包覆在鐵-鋅片上、不含銀之鐵-鋅片以及銀板的聚合物進行抗菌功效測定。第二，抗風化試驗由鐵-鋅片包覆銀塗料在25℃與相對濕度為85％環境下，檢測不同時間的耐久度。第三，消耗率檢測由鐵-鋅片包覆銀塗料與10％的聚環氧乙烷（PEO）反應，不同的反應時間由大腸桿菌存活數量檢測鐵-鋅片包覆銀塗料的消耗率。第四同樣於第三設計，由工研院分別加入兩次較高濃度大腸桿菌，檢測抑制與消耗能力。接著我們也檢測鐵-鋅片包覆銀塗料對於醫院五個不同位置天花板採集樣本進行抗菌測試，同時我們調查此材料對人包皮纖維細（Hs68）的細胞毒性。最後，比較聚乙烯（PE）包覆銀塗料和PE材料針對大腸桿菌和金黃色葡萄球菌抗菌效果。使用Ag塗料製成鐵-鋅板增強抗菌功效。鐵-鋅片包覆銀塗料能保持良好抗菌效果至少60天。甚至在90天後噴灑大腸桿菌於表面仍保持抗菌效果。再保存154天後Ag塗料板比使用Co或Ni塗料板還有更好的抗菌效果。以鐵-鋅片包覆銀塗料與細胞培養液反應1小時候再培養細胞，發現這些材料對細胞是無毒性的。然而，Hs68細胞的細胞生存力再與溶液培養24小時後開始下降。Ag塗料包覆的材料和PE材料顯示對大腸桿菌和金黃色葡萄球菌有最佳的抗菌活性。

關鍵字

抗菌效能 ； 生物毒性研究 ； 高價金屬

並列摘要

Antibacterial efficacy of products can be improved by coating silver (Ag) with polymers. Four designs have been planned in the current study. First, the antibacterial efficacy of the Ag-coated iron-zinc plates using different polymers as adhesive, the plates without Ag, and the plates with Ag but without using polymers were determined. Second, weathering resistance tests were performed on the Ag-coated iron-zinc plates by polymers after storage at 250C and 85% of relative humidity for different periods of time. Third, the consumption rates of the Ag-coated iron-zinc plates by 10% PEO (Polyethylene oxide) after different periods of time were determined by survival of Escherichia coli adding to surface of the iron-zinc plates before storage. Fourth, the consumption rates of the Ag-coated iron-zinc plates by 10% PEO can also be determined by the ability of plates to inhibit the growth of E. coli after spraying E. coli by ITRI (Industrial Technology Research Institute) then storage and inoculated a higher concentration of E. coli in our lab afterward. Next, the antibacterial efficacy of different materials-coated iron-zinc plates on the ceilings above five different locations in a hospital at time points also assessed. We investigate the cytotoxicity of materials tested in human foreskin fibroblast cells (Hs68). Last, the antibacterial activity of the paint also PE (Polyethylene) materials with Ag, the paint and PE materials only against E. coli and S. aureus. The usages of polymer made the addition of Ag on the surface of iron-zinc plates stable and enhance the antibacterial efficacy of the plates. The iron-zinc plates with Ag can maintain their antibacterial abilities for at least 60 days after storage. Even there was E. coli sprayed on the surface, the iron-zinc plates with Ag can still maintain their antibacterial abilities for at least 90 days after storage. The locations using Ag-coated plates showed better antibacterial activity for 154 days than those using Co or Ni-coated plates. No cytotoxicity of the tested materials was observed when the cells were incubated with the solution containing detached materials for 1 hour. However, the cell viability of Hs68 cells was decreased after the 24-hours incubation with the solution used to immerse the tested materials for 24 hours. The addition of Ag to paint and PE materials showed the best antibacterial activity against E. coli and S. aureus.

並列關鍵字

antibacterial efficacy ； biological evaluation ； high-valence silver material

參考文獻

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