- 學位論文
噴射式低溫大氣電漿應用於生物膜滅活之研究
The study of inactivation biofilm by Cold Atmospheric Pressure Plasma Jet
摘要
細菌主要以生物膜菌體的形態存在於自然界，而懸浮菌體形成生物膜後對於環境的耐受性會相對提高，導致難以滅除，因此需要新的滅菌方式。低溫大氣電漿近年來已經廣泛的運用於生物醫學方面，主要優勢為對細菌無專一性以及對人體無熱效應或是無電刺激。目前大多電漿滅菌研究都以懸浮菌體為主，因此本研究將探討噴射式低溫大氣電漿對於生物膜之影響。本研究利用自行製作之電源供應器與電漿反應器做為滅菌研究。藉由電源供應器所提供之10.6 kV工作電壓下與102.4 kHz工作頻率下激發電漿，其消耗功率為24.29 W，電漿長度為35 mm，電漿激發20分鐘後溫度仍低於45.5℃。利用光學放射光譜儀檢測本研究之電漿可於391.32 nm、581.88 nm、706.21 nm觀察到氦氣放射光譜；297.62 nm、309 nm、777.32 nm則可觀察到氧氣的光譜。本研究電漿系統最大滅菌範圍為1.03〖 cm〗^2。在生物膜培養方面，每片培養出的生物膜試片細菌濃度大約位於107~108 CFU/ml間，且具有良好的穩定性。進行生物膜滅活之定量實驗，同時利用雷射共焦掃描顯微鏡分析電漿可侵入生物膜厚度為36.2 μm，從場發射掃描式電子顯微鏡結果發現，細菌經過電漿處理過後，在表面形貌產生極大變化。
並列摘要
It is accepted that biofilms are the predominant phenotype of most bacteria both in natural settings and more resistant than planktonic phenotype, so the urgent need for new antimicrobial strategies. In recent years, cold atmospheric pressure plasma has been widely used in biomedical applications. It would not cause any thermal effects or electrical stimulation. Most study of sterilization are focused on planktonic phenotype. Thus, in this study we will use homemade power supply and plasma jet device for sterilization. In the same time, we investigate the influence of plasma for biofilm by laser scanning confocal microscopy. The applied voltage and frequency of the damping waveform is respectively 10.6 kV and 102.4 kHz, the total power of plasma jet is 24.29 W; The maximum plasma plume and applied temperature is respectively 35 mm and below 45.5℃. The emission spectrum of helium can be observed at 391.32 nm, 581.88 nm and 706.21 nm; The spectrum of oxygen can be observed at 297.62 nm, 309 nm and 777.32 nm; The largest bacteria growth inhibition area by plasma reach to 1.03 cm2; The bacterial concentration of biofilm samples are about 107~108 colony-forming unit (CFU)/ml; The plasma can penetrate through the biofilm reaching 36.2 μm; After plasma treatment,we can be observed that regular structure of the biofilm was destroyed and replaced with ruptured bacteria.