電漿誘導聚酯纖維鍵結幾丁聚醣形成具抗菌功能性之表面

Plasma Induced Chitosan Immobilization on the PET Fiber to Form an Antibacterial Surface

林明哲(Ming-Jer Lin)

指導教授 ： 廖峻德

中原大學/工學院/醫學工程研究所/碩士(2003年)

https://doi.org/10.6840/cycu200300531

摘要

摘 要
本研究以聚酯纖維(PET)為基材利用微波氧氣電漿活化，使表面產生過氧化物，而後結合熱與氧化還原分解反應來接枝丙烯酸單體，再利用丙烯酸的羧基，使之與幾丁聚醣的羥基或銨基形成酯化或醯胺共價鍵結，完成固定幾丁聚醣的程序。接著以Reactive Red 239做染色反應，利用紫外線/可見光光譜儀於最大吸收波長541 nm處量測其吸收值進行定量分析；並將試片以減弱式全反射-傅立葉轉換紅外光譜儀、電子能譜化學分析儀，對改質基材進行表面化學元素及鍵結的分析另以掃瞄式電子顯微鏡觀察改質基材表面結構變化。本研究之抗菌評估方面除測試幾丁聚醣之抗菌活性外更進一步以JIS L 1902之纖維製品抗菌、抑菌測試法為準則，做試片殺菌、抑菌能力之定量測試。隨後將改質後的試片依據CNS-8038之家庭用洗衣機洗濯法進行抗菌水洗堅牢度。實驗結果顯示：幾丁聚醣的固定量約在56±3.53μg•cm-2；而在表面分析中,證明幾丁聚醣與接枝上的丙烯酸是藉由醯胺及酯化鍵結反應結合；在幾丁聚醣之抗菌活性測試中發現，幾丁聚醣對金黃色葡萄球菌、表面葡萄球菌、大腸桿菌、 綠膿桿菌均具有相當優異的殺菌與抑菌效果；而在試片測試則發現：試片的抑菌活性值大於JIS L 1902測試法的標準值，而其殺菌活性值亦大於標準規範值，顯示所固定上的幾丁聚醣，仍保有本身的殺菌、抑菌能力；抗菌耐水洗滌測試結果顯示：試片接枝丙烯酸之PET纖維在清洗6次後及鍵結幾丁聚醣之PET纖維在清洗10次後仍然對金黃色葡萄球菌、表面葡萄球菌、大腸桿菌、綠膿桿菌具有抗菌效果。

關鍵字

幾丁聚醣 ； 丙烯酸單體 ； 微波氧氣電漿 ； 抗菌效果。 ； 聚酯纖維

並列摘要

Abstract

In this study, polyethylene terephthalate (PET) fabric was pretreated using antenna coupling O2 microwave plasma to improve surface activation, followed by grafting with acrylic acid (AAc) monomer and generating O=C-O group at surface. The pAAc-grafted PET was then given ester and amide covalently bonded with chitosan, under varied immobilization process. The chitosan-immobilized substrates were reacted with C.I. Reactive Red 239, using UV/VIS absorbance measured at 541 nm for quantitative analysis. Applying Fourier-Transformed Infrared Spectroscopy with Attenuated Total Reflection (FTIR-ATR), Electron Spectroscopy for Chemical Analysis and Scanning Electron Microscopy (SEM), we examined the chemical structures and the element function abilities of the modified surfaces. The JIS L 1902 testing method for fabric products was applied for the chitoson-immobilized samples on the antibacterial capability tests. The CNS 8038 testing method for fabric products was applied for the chitoson-immobilized samples on the water fastness of antibacterial capability tests. Experimental result has demonstrated that the chitosan-immobilized quantity was ca. 56±3.53μg•cm-2, while the linkage between AAc and chitosan was confirmed as expected as the amide and the ester bond. The antibacterial activities test of different deacetylation chitosans for E. coli , Staph. Aureus, Staphylococcus aureus and Staphylococcus epidermidis provided an excellent antimicrobial effect and pasteurization effect. The antibacterial activities test of these samples show both of the antimicrobial value and the pasteurization value were over the JIS L1902 standard value. It revealed that the immobilized chitosan maintained its capability to inhibit bacterial proliferation. According to the CNS-8038 method the PET grafted AAc was washed about six times and PET (grafted AAc) immobilized chitosan was washed more than ten times, the surface still have antibacterial ability

並列關鍵字

Polyethylene terephthalate fabric ； Acrylic acid ； O2 microwave plasma

參考文獻

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