神經組織受超音波刺激後之反應探討

Studies on the Response of Neural Tissues following Ultrasonic Stimulations

崔博翔(Bor Shyang Tsuei)

指導教授 ： 王士豪

中原大學/工學院/醫學工程研究所/碩士(2001年)

https://doi.org/10.6840/cycu200100393

摘要

摘 要

根據過去之研究文獻記載，當超音波作用在生物組織上，音波的傳動促使組織分子產生熱、振動或按摩等生物效應，並具有建設性與破壞性的物理治療效果。為使超音波廣泛的運用於物理治療，因此超音波對於神經肌肉組織的影響機制需先被探討。

本研究以牛蛙之坐骨神經做為實驗材料，手術取下坐骨神經，並將其放置在神經紀錄槽進行體外刺激實驗(in vitro)。首先使用電刺激器產生電訊號刺激神經組織，以誘發神經組織之複合動作電位做為對照組。再利用超音波刺激系統產生不同能量之超音波施加於神經組織上，並與對照組比較，以探討神經組織於超音波刺激前後，複合動作電位之變化情形，由此評估不同能量超音波刺激對於神經組織之影響機制。

透過神經複合動作電位的相關係數、振幅、以及神經傳導速率之分析結果可以發現，當施加超音波刺激於神經組織，可降低刺激前後神經複合動作電位之相關性，刺激能量越大，相關程度越低。此外不同能量之超音波刺激神經組織，不僅可促進或抑制複合動作電位振幅的上升或下降，尚可使神經組織之傳導速率增加。以實驗結果推論，超音波效應可影響神經組織離子通道閘門開啟或關閉之機制，使得細胞膜本身之離子通透性發生改變，進而改變神經複合動作電位之振幅與傳導速率。

關鍵詞: 超音波生物效應、神經複合動作電位、神經傳導速率

關鍵字

神經複合動作電位 ； 神經傳導速率 ； 超音波生物效應

並列摘要

Abstract

When an ultrasonic wave propagates through a biological tissue, bioeffects including those induced by heat and vibration could result a constructive or destructiveness effect for physiotherapies according to previous studies. To better apply ultrasound on physiotherapies, the mechanisms about the response of nervous and muscular tissues following ultrasonic stimulations should be explored thoroughly.

This study investigates the effects of ultrasonic stimulations on the sciatic nerve of the bullfrog suspended in the nerve chamber in vitro. Firstly, the compound action potential (CAP) of the nervous tissue evoked by electric stimulations was collected for the control group. Subsequently, the nervous tissue was stimulated with different ultrasonic intensities. Changes of the CAP of the nervous tissue following ultrasonic stimulations were then processed to evaluate the mechanism and influence of the response between different ultrasonic intensities stimulations and the nervous tissue.

The experimental results were analyzed using the correlation coefficient, amplitudes, and conduction velocity of the CAP signals collected before and after ultrasonic stimulations. The correlation coefficient was found to be reduced after ultrasonic stimulations. The more intensity was employed, the lower correlation coefficient was obtained. On the other hand, stimulating the nervous tissue with different ultrasonic intensities not only enhance or reduce the amplitude of the CAP, but also raise the conduction velocity of the nervous tissue. These were postulated due to that ultrasound could affect the mechanism of opening and closing ion channel gate causing modifications of ion permeability in the cell membrane.

Keyword: Ultrasound bioeffect , Compound action potential , Conduction velocity

並列關鍵字

Conduction velocity ； Ultrasound bioeffect ； Compound action potential

參考文獻

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