- 學位論文
固定幾丁聚醣聚丙烯不織布在抗菌與醫療上的應用
Immobilized Chitosan onto Polypropylene Non-Woven Fabric Surface for Anti-bacterial and Biomedical Applications
摘要
本研究乃是將聚丙烯不織布經表面改質後，進一步固定上幾丁聚醣；並評估所製成之試片應用於醫療創傷使用的可行性。因此本研究共分為三個部分：定量定性分析、抗菌測試、及血液相容性測試。 利用陣列天線式氧氣低溫微波電漿進行聚丙烯不織布表面活化，經過數秒的電漿處理，在其表面大量地產生氧化基團。而後結合熱與氧化還原分解反應來接枝丙烯酸單體，再利用EDC-NHS活化丙烯酸的羧基(O=C-OH)使之與幾丁聚醣的胺基(-NH2)形成共價的醯胺鍵結(O=C-NH)。經固定幾丁聚醣的試片與CBR 3B-A反應，使用紫外線/可見光光譜儀於波長575 nm處的吸收值以進行定量分析；並以減弱式全反射-傅立葉轉換紅外光譜儀、電子能譜化學分析儀，對改質基材表面進行化學元素及鍵結的分析。之後，以JIS L 1902纖維製品試驗法對固定幾丁聚醣的試片做殺菌、抑菌能力的測試；並使試片與血漿接觸，量測培養後血漿的部分活化凝血時間、凝血時間的時間改變、及纖維蛋白的濃度變化；同時也計算紅血球、血小板及白血球在固定幾丁聚醣表面上的吸附率，以評估其生物活性。利用掃描式電子顯微鏡取得與血液接觸培養後的試片之外觀型態。實驗結果顯示：幾丁聚醣的固定量為47.91±2.51 μg·cm-2，幾丁聚醣與聚丙烯不織布上的丙烯酸單體，如預期是藉由醯胺所鍵結。其抑菌活性值，皆大於JIS L 1902測試法所規定之標準值2.2，而其殺菌活性值亦大於標準規範值0，顯示所固定上的幾丁聚醣，仍保有本身的殺菌、抑菌的能力。由血液凝固測試知：試片與血漿培養後，幾丁聚醣並不會影響血液的內在凝血路徑；然而，進一步於全血測試中，幾丁聚醣則具有吸附紅血球、血小板及白血球的能力。此現象與幾丁聚醣的原有特性有關。
並列摘要
Polypropylene (PP) non-woven fabric was surface-modified and subsequent to immobilize chitosan. Feasibility of applying such samples to wound dressing related uses was evaluated. The work was separated into three parts: qualitative and quantitative studies, anti-bacterial tests, and blood compatibility tests. Low-temperature antenna-array O2 microwave plasma was utilized for the activation of non-woven PP fabric. With several seconds of plasma treatment, oxidative groups were massively created at surface. Using thermal and reduction-oxidation reactions, the diluted acrylic acid (AAc) was subsequently graft-copolymerized. Chitosan was then immobilized on the fabric using EDC-NHS as coupling agents to originate O=C-OH (from the functional group of AAc) and –NH2 (from the side group of chitosan) and to form covalently the amide bonds (O=C-NH). The chitosan-immobilized substrates were reacted with CBR 3B-A, using UV/VIS absorbance measured at 575 nm for quantitative analysis. Applying Fourier-Transformed Infrared with Attenuated Total Reflection and Electron Spectroscopy for Chemical Analysis, we examined the chemical structures and the element functionabilities of the modified surfaces. The JIS L 1902 testing method for fabric products was applied for the chitoson-immobilized samples on the anti-bacterial capability tests. Such modified samples were then contacted with blood plasma for the assessment of activated partial thromboplastin time, thrombin time, and fibrinogen concentration tests. Simultaneously, the absorption rates of red blood cells, platelets and white blood cells on the chitosan-immobilized surfaces were calculated to assess their bioactivities. Scanning Electron Microscopy was used to take the morphologies of the blood-contacted sample. Experimental result has demonstrated that the chitosan-immobilized quantity was ca. 47.91±2.51 μg·cm-2, while the linkage between AAc and chitosan was confirmed as expected as the amide bond. The anti-bacterial index of the sample was over the standard value, 2.2, whereas the index related to the capability of bacteria inhibition was higher than the standard value, 0. It revealed that the immobilized chitosan maintained its capability to inhibit bacterial proliferation. From the blood clotting tests, the chitosan-immobilized surface did not affect the intrinsic clotting pathway. Nevertheless, the surface was capable to adsorb red blood cells, platelets and white blood cells in the blood tests. Furthermore, red blood cells accumulated on the chitosan-immobilized surface was observed using SEM. These phenomena could be referred to the characteristic of chitosan itself.