化學修飾網版印刷電極於轉胺酶檢測的應用

Chemically Modified Screen-Printed Electrode for Transaminases Determination

歐陽梅(Mei Ou-Yang)

指導教授 ： 吳吉輝

中原大學/理學院/化學研究所/碩士(2003年)

https://doi.org/10.6840/cycu200300692

摘要

中文摘要
近年來化學修飾電極在電化學的偵測上，因易受到檢體成份的干擾，又由於傳統電極的造價昂貴，故分析家以網版印刷電極取代一般的傳統電極。本研究是利用物理吸附的方式將塗覆物質包括 Meldola’s Blue、麩胺酸去氫酶、輔酶NAD+及羧甲酸纖維素介面活性劑固定在網版印刷電極的表面，製備成化學修飾酵素網版印刷電極電極-- MB+-NAD+-GLDH / SPE。利用Chronocoulometry電化學法測試(1)MB+修飾網版電極以及(2)MB+-NAD+-GLDH修飾酵素網版印刷電極。探討了(1)電極修飾物的選擇、(2)檢測電位的選擇、(3)避開干擾物(尿酸、抗壞血酸)的探討、(4)測試轉胺酶ALT的活性、(5)反應物的濃度以及(6)檢測參數。

此電極分析NADH和Glutamate 的偵測極限為5x10-6M；檢測轉胺酶時間為20秒，分別可測得活性範圍為ALT是5U/L~200U/L ，AST是5U/L~60U/L。

關鍵字

菸鹼醯胺核苷酸 ； 網版印刷電極 ； 麩胺酸鹽

並列摘要

Abstract
In electrochemical analysis, chemically modified electrodes are found to be easily contaminated by sample to be analyzed and conventional electrodes are expensive, therefore In recent year, screen-printing electrodes are developed to substitute for them.

In this study, Meldola’s Blue, Glutamate dehydrogenase, nicotinamide adenine dinucleotide (NAD+) as the cofactor and Carboxymethylcellulose surfactant（CMC）are mixed and immobilized by physical adsorption on the screen-printing electrode to prepare the MB+-NAD+-GLDH/SPE chemically modified electrodes.

Chronocoulometry method is used to assay (1) MB+-CMC /SPE and (2) MB+-NAD+-GLDH /SPE. In addition, (1) Modifier for the electrodes (2) potential applied (3) exclusion of interference by uric acid and ascorbate (4) transaminase activity (5) concentration of reactant and (6) assay parameters are studied.

The detect limits of MB+-NAD+-GLDH /SPE for NADH and Glutamate are 5x10-6M, respectively. And the MB+-NAD+-GLDH /SPE gave linear response in the range of 5-200U/L for ALT activity and 5-60U/L for AST activity, respectively. It takes 20 seconds to measure the concentration of transaminase.

並列關鍵字

NADH ； Glutamate ； Screen-Printed Electrode

參考文獻

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