電化學式麩胺酸丙酮酸轉胺酶及麩胺酸草醯乙酸轉胺酶生物感測器之開發研究

Studies on the Development of an Electrochemical Glutamate pyruvate transaminase & Glutamate oxaloacetate transaminase Biosensor

黃鏗元(Keng-Yuan Huang)

指導教授 ： 陳建源

國立臺灣大學/生命科學院/微生物與生化學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.10231

摘要

本研究是利用酵素能夠藉辨別獨特的分子特性，使用L-麩胺酸氧化酶做為感測器系統之生物化學反應的感測元件，構築檢測肝弁鄎

關鍵字

生物感測器 ； 化學修飾電極 ； L-麩胺酸感測器

並列摘要

An electrochemical enzymatic biosensor is based upon the characteristic of an enzyme capable to recognize a particular molecule through an oxidation-reduction effect. In this study, the Glutamate oxidase is utilized as the sensing components of our sensor system to initiate biological and subsequent electrochemical reactions. The characteristic current-time curves are fairly interpreted using three types of calculation models including end-point measurement, quasi-kinetic measurement, and quasi-kinetic measurementⅡ. The end-point measurement is adopted in this study. For the purpose of ezyme-immobilization, polyvinylpyrrodine is utilized to embed HRP、K3Fe(CN)6, and Glutamate oxidase on the screen-printing electrode to prepare the HRP/K3Fe(CN)6/GMOx/SPE chemically modified electrode.
The optimal substrate composition for the determination of GOT activity is 1mM

並列關鍵字

L-Glutamate ； Amperometric biosensor ； Immobilized

參考文獻

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