本研究是利用酵素能夠識別特定分子的特性,並且催化這些分子進行特殊的氧化反應,形成某特定物質之增減。當產生電化學反應可使該特定物質的變化轉換成電訊號,再利用訊號的差異表現來表示酵素的消耗與特定分子定量間的關係。對於進行可自然發生反應,需要提供一能量給反應物,經研究設定固定電壓為0.6伏特以提供能量。固定電壓法在設計上包含了工作電極及參考電極,在工作電極上的電位值由參考電極所控制。利用工作電極上的固定電位,量測因酵素電極產生不同程度反應的電流量。藉由測定三種因素:工作電壓、酵素反應的酸鹼值及血糖濃度的偵測範圍,則此反應所產生的電壓或電流量可量測出。並利用以上結果對市售電極片做電壓產生特性及量測品質的評估。本研究在量測血糖濃度時,設定濃度介於100mg/dl與290mg/dl之間,均可以0.6伏特作為最佳之工作電壓。以此適當範圍對生理酸鹼值6.6至7.6之間實驗,測出酸鹼值對電壓對時間變化並無明顯差異。同理,藉由上述條件以研究葡萄糖濃度影響因子,實驗結果顯示:葡萄糖濃度對電壓產生的變化有顯著差異且具有高度重現性,因此可應用到製作與設計血糖儀。在訊號讀取的設定上,由實驗得到測試時間在35秒至70秒間電壓對時間變化曲線呈線性,為避免放大器之截止時間相近,在快速檢測的原則下選擇以40秒作測量時間。基於對上述因子的研究,針對市售商品R牌、B牌及A牌做評估:在一定時間內的變化量中,以R牌的電壓對時間變化最大,且在相關係數中R牌 以0.90至0.99接近線性的程度最好。本研究因此提供自製或篩選葡萄糖感測電極時的一種快速品質評估方法。
This work is based upon the characteristic of an enzyme capable to recognize specific molecules, to promote their oxidative reactions, and to provoke quantitative changes of a particular reactant or product. The extent of substance can be furthermore transformed into electrical signals when electrochemical reactions occur. Variation of signals can be expressed as the relation between the consummation of enzyme and the quantity of the specific molecule. For a spontaneous process, a required energy is provided to the reactants, the applied voltage for the energy is studied and fixed at 0.6V. This voltage supply system includes a working electrode and a reference electrode. The reference electrode controls the potential at the working electrode, which records electrical currents from varied enzymatic reactions occurred at the electrode. By evaluating three dependent parameters: working voltage of enzyme electrode, pH value of enzymatic reactions and detecting range of glucose concentration, the voltage or current derivative from the reaction can be measured. This study is also extended to commercially available electrodes and applied to assess their output voltage and quality of measurement. The appropriate detecting range of glucose concentration is optimized in the range of 100-290mg/dl. The most sensible working voltage is fixed at 0.6V. With these detecting levels, the pH value in the range of 6.6-7.6 for enzymatic reactions does not significantly affect DV/Dt (the change of measured voltage with respect to the measuring time). Similarly, we examine the influential factor of glucose concentration to DV/Dt, a significant difference is obtained and the result is highly reproducible. It is therefore applicable to design or to make glucose sensors. The suitable measuring duration is set in the range of 35-70sec, when DV/Dt is approximately linear. To avoid electrical disturbance and to keep a quick response, a measuring time of 40sec is optimized. Based upon these factorial studies, the commercially available electrodes, R、B及A, are assessed. Within the measuring time, the DV/Dt for R is highest and its correlation coefficient is around 0.90-0.99. The result of this study is therefore valuable to provide a quick quality assessment for making as well as selecting glucose sensors.