- 學位論文
量測微循環冷熱溫度效應之系統研製
A measurement system for temperature effect on microcirculation
摘要
微循環的主要作用是維持所有組織細胞的正常代謝和功能，因而維繫著人體生命的重要生理作用。為了透過對微循環的動態觀察以得到有關身體內部情況的訊息，本研究整合了光學機構、光電檢測線路與程式軟體，建立了一部雷射都卜勒系統來量測組織微循環在受到溫度變化時的反應情形。本系統使用Y型光纖傳導光源並接收反射光訊號，以相同的架構同時量測都卜勒血流訊號以及脈動血量訊號，並且利用致冷晶片改變皮膚組織表面溫度來影響其微血管循環，最後使用資料擷取卡將類比訊號轉成數位訊號經由程式軟體處理，即時顯示出血流與血量訊號變化。利用本研究所研製之系統實際量測健康人體之結果顯示，紅光雷射可以清楚測得血流訊號，但無法測得微小的血量變化，而使用綠光雷射則可同時測得微小之血量與血流的變化，且紅光的血流訊號變化幅度大於綠光的訊號。在綠光血量訊號方面，拇指以及前手臂部位受到冷刺激時皆呈現下降趨勢，反之，在加熱時則皆有上升趨勢，可是血流訊號方面，拇指部位在冷卻時則有訊號上升，而加熱時訊號下降的情形，其原因可能是動靜脈吻合血管調節表淺之微循環所造成。研究中並對血流與血量訊號訂定各種量化參數，以便將循環對溫度變化的反應進行量化分析比較，這些量化參數將有助於判斷微循環障礙以及血管病變之情況。
並列摘要
Microcirculation is crucial to maintain the cell function. The condition of microcirculation can also provide some useful physiological information. In this study, a laser measurement system was constructed to detect the temperature effect on microcirculation in skin tissue. The measurement system includes optical system, optoelectronic detecting circuit, and control software. Laser light is guided by a bifurcated optical fiber bundle to the measurement site, and the reflected light is collected and sent to the photo-detector through the second branch. The reflected light includes the high frequency laser Doppler signal and the low frequency blood pulse signal. The signal is acquired by a data acquisition board and analyzed for real time monitoring. A thermoelectric cooler is also built inside the probe to alter skin temperature. The measurement results on healthy subjects show that although the red light laser can easily measure the blood velocity, but it is not suitable for measuring the small change in blood volume. The green light laser can measure both the blood flow velocity and blood volume signals of skin tissue. The blood volume signals on finger tip and forearm decrease with cooling and rise with heating. In order to quantify the temperature effect of microcirculation, several parameters are defined on the dynamic change of blood flow velocity and blood volume signals with thermal stimulation. These parameter might be useful to identify some pathological conditions of microcirculation.