利用高果糖誘發大鼠代謝症候群模式探討複方食材對代謝症候群的影響

Investigating the Effects of Combinatorial Foods on Metabolic Syndrome in A Fructose – Fed Rat Model

陳佳慧(Jia-Hui chen)

指導教授 ： 吳亮宜

中原大學/工學院/生物科技研究所/碩士(2009年)

https://doi.org/10.6840/cycu200901286

摘要

代謝症候群是由數種代謝異常結合於同一人身上的疾病。研究指出，代謝症候群的患者，得到糖尿病、心臟病的機率與死亡率會大幅增加。故本實驗將利用複方食材，評估其改善代謝症候群之功效。
實驗設計將雄性SD大鼠分成四組，分別是空白組 (B)：餵飼50%玉米澱粉和10%蔗糖混和飼料；控制組 (C)：餵飼60%高果糖飼料；食材處理組1 (T1)：餵飼高果糖飼料，並添加紅麴、大豆、綠藻、苦瓜及甘草複方。食材處理組2 (T2)：同樣餵飼高果糖飼料，並添加兒茶素、豆豉、鰹魚及綠藻複方。實驗持續進行13週。實驗期間分析血壓、禁食血漿血糖、三酸甘油酯 (TG)、總膽固醇 (TC)、高密度脂蛋白膽固醇 (HDL-C)、低密度脂蛋白膽固醇 (LDL-C) 及與胰島素訊息傳遞相關蛋白之磷酸化活性表現量。實驗期間並進行口服葡萄糖耐受性測試，觀察大鼠胰島素敏感性之變化。
實驗結果顯示，C組之血壓、TC、TG、LDL-C濃度、肝臟總膽醇濃度皆顯著較B組高。而胰島素敏感性與高密度脂蛋白膽固醇比例 (HDL-C/TC) 則顯著低於 B 組，代表C組產生了代謝症候群之現象。探討其機制，C組之insulin receptor (IR) 及Akt的磷酸化量和Glucose transportor 4 (Glut 4) 表現量與B組比較皆有下降的趨勢。相較於C組，T1組具有較高的HDL-C/TC和胰島素敏感性，較低的血壓、TG與腹部脂肪。而其IR、insulin receptor substrate-1 (IRS-1)、Akt及Glut 4之表現量和Akt活化量與C組比較皆有上升的趨勢。T2組則有較高的HDL-C/TC、胰島素敏感性，與較低的血壓、三酸甘油酯和總膽固醇濃。且其IR、Akt及Glut 4之表現量和Akt活化量與C組比較亦皆有上升的趨勢。顯示本實驗使用之複方食材可有效改善代謝症候群之症狀。
另本實驗中所使用的空白組飼料非一般常用的 chow diet，因此，我們另設計一實驗比較兩種飼料的大鼠的影響。實驗設計將雄性SD大鼠分成兩組，分別是實驗組 (Co)：餵飼餵飼50%玉米澱粉和10%蔗糖混和飼料；對照組 (Ch)：餵食chow diet。實驗結果顯示，Co組之腹部脂肪、TC、TG及胰島素濃度皆顯著較Ch組高。推測原因可能與微量礦物質、脂質、脂肪酸種類及蔗糖有關。

關鍵字

代謝症候群 ； 高果糖 ； 複方食材

並列摘要

The metabolic syndrome is a common metabolic disorder on the person body. The previous study showed that the presence of the syndrome predicted increased cardiovascular disease and coronary heart disease mortality. The objective of this study was to evaluate the effects of the supplementation of the combinatorial health food on metabolic syndrome in a fructose-fed rat model.
The male SD rats were divided into four group: blank group (B), fed with 50% corn starch + 10% sucrose diet ; control group (C), fed with high fructose diet alone ; treatment group 1 (T1), fed with high fructose diet plus red yeast rice, soybean, bitter melon, licorice and green algae; treatment group 2 (T2), fed with high fructose diet plus green algae, katsuobushi oligopeptide, catechin, and touchi extract. The study lasted for 13 weeks. Biochemical parameters related to blood pressure, fasting plasma glucose, insulin, triglyceride, total cholesterol, abdominal fat mass and the phosphorylation activity and expression of insulin signaling pathway related proteins were measured, and an oral glucose tolerance test (OGTT) was performed in this study.
The data shown that group C had significant increase in plasma triglyceride, cholesterol, LDL-C, hepatic total cholesterol and systolic blood pressure. Insulin sensitivity and HDL/TC ratio were decrease as compared to group B. Further evaluated the possible mechanism of high fructose diet induced metabolic defects, we found that the rats showed decreased phosphorylation activities of insulin receptor (IR) and Akt and total content of glucose transporter IV (Glut 4).
Compairing with group C, group T1 had higher HDL-C/TC ratio, and insulin sensitivity, and lower systolic blood pressure, triglyceride and abdominal fat mass. And the expressions of IR, insulin receptor substrates-1 (IRS-1), Akt and Glut 4 and phosphorylation activities of Akt were raised in T1 group.
Group T2 had lower systolic blood pressure, triglyceride and TC level as compared to group C. And the decreased insulin sensitivity was also ameliorated in group T2, which might result from the increased expressure of IR, Akt and Glut 4 and the increased phosphorylation activity of Akt.
The result showed that the combinatorial health food we used in this study may improve of metabolic syndrome effectively. Furthermore, the basal diet we used in the above was not the chow diet in common use. We designed anther study to investigate the influence of two diets on rats.
The male SD rats were divided into two group: experimental group (Co), fed with 50% corn starch + 10% sucrose diet ; control group (Ch), fed chow diet alone. The data shown that group Co had significant increase in plasma triglyceride, cholesterol, insulin, insulin and abdominal mass fat. And we suggested these phenomena induced by group Co may attribute to the contents of micro-mineral, sucrose and lipid.

並列關鍵字

metabolic syndrome ； high fructose ； combinatorial food

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