- 學位論文
發光二極體光照刺激對高血糖鼠燒燙傷口分泌表皮生長因子的影響
Effects of LED Light Irradiation on the Secretion of EGF from the Burns of Hyperglycemic Rats
摘要
本研究為探討糖尿病是否會對表皮生長因子(epidermal growth factor, EGF)的分泌所造成影響，並且在傷口癒合過程中加入光照刺激的條件，觀察是否能促進EGF之分泌，進而達到加速傷口癒合的效果。實驗選用Wistar大鼠作為實驗動物模型，實驗組別分為(1)燙傷組(2)燙傷照光組(3)高血糖燙傷組(4)高血糖燙傷照光組。在各組老鼠背部製造1x1平方公分的傷口，接著使用發光二極體(波長630-nm、平均功率14-mW/cm2)對照光組別的傷口進行相隔48小時一次、每次280秒的光照處理。並做EGF生長因子分析、傷口外觀觀察與組織切片來評估傷口癒合狀況。 實驗結果發現，使用630-nm LED光照刺激正常鼠及高血糖鼠燙傷傷口，可以促使傷口外觀面積縮小。在使用Elisa Kit檢測EGF濃度部分，燙傷照光組於燙傷後第2天開始大量增加，相較於燙傷組提早了兩天到達峰值，而高血糖燙傷照光組初期所測得的EGF濃度相對於高血糖燙傷組高，且趨勢與燙傷組相似。實驗結果表示，630-nm LED光刺激能促進傷口中的EGF提早分泌，而達到加速傷口癒合的效果。
並列摘要
The aim of this study was to investigate effect of light-emitting diode (LED) light irradiation on the secretion of epidermal growth factor(EGF) from the burns of hyperglycemic rats. This study used Wistar rats. The rats were divided into four groups: 1. Burned 2. Burned+LED 3. Hyperglycemic(Hyper.)+Burned 4.Hyper. Burned + LED. Rats received intraperitoneal injections of Alloxan monohydrate (90 mg/kg) with a blood glucose of 300 mg/dL. The wounds(1x1cm2) on the back of the rats were treated with LED (630 nm, 14 mW/cm2, 280 sec, total energy 3.92 J/cm2, once every two days). Wound healing were evaluated by the concentration of EGF, observe the appearance of the wound and histological evaluation. Our results showed that LED-treated promote reducing wound area in wound healing process. The concentration of EGF in group 2 is reaches a peak two days earlier than the group 1. The trend of group 4 and group 1 are similar. However, there is no significant differences in secretion statistically (P>0.05). In conclusion, we provide evidence, 630-nm LED light seems to promote the EGF stimulation peaked earlier, to achieve the effect of accelerating wound healing.