發光二極體光照刺激對高血糖鼠燒燙傷口分泌血小板衍生性生長因子的影響

Effects of LED Light Irradiation on the Secretion of PDGF from the Burns of Hyperglycemic Rats

紀慧儀(Hui-Yi Chi)

指導教授 ： 謝瑞香

中原大學/工學院/生物醫學工程研究所/碩士(2012年)

https://doi.org/10.6840/cycu201300165

摘要

本研究目的為探討發光二極體(Light Emitting Diode, LED)光照刺激高血糖鼠燙傷傷口影響，進而瞭解血小板衍生生長因子(Platelet-Derived Growth Factor BB, PDGF-BB)的分泌量與其分泌細胞間的相關性。研究使用Wistar大鼠，總共分四組。老鼠空腹注射Alloxan monohydrate (80~100 mg/kg)誘發成為高血糖鼠。燙傷面積1×1 cm2。LED參數為波長630 nm、總能量為 4 J/cm2，兩天照光乙次。分析方法採用組織學和PDGF-BB濃度檢測。從組織學結果發現，LED光照能使正常鼠傷口的巨噬細胞、纖維母細胞和血管生成最大值提早2天出現；而高血糖鼠巨噬細胞和血管生成的最大值也會提早2天，纖維母細胞則提早了4天。接著由PDGF-BB分析結果發現，正常鼠燙傷經LED光照後能使PDGF-BB分泌提早，且於11天以前效果較顯著。高血糖鼠方面，其傷口PDGF-BB含量會較正常鼠來得少；然而透過LED照射能讓PDGF-BB提前分泌。由研究結果得知LED光照能促進正常鼠和高血糖鼠的巨噬細胞、纖維母細胞最大量提早出現以及加速血管生成，進而促進其PDGF-BB的分泌，又以8天以前效果較好。

關鍵字

發光二極體 ； 高血糖鼠 ； 血小板衍生性生長因子

並列摘要

The aim of study was to determine the correlation for healing burn wound of hyperglycemic rats by light-emitting diode (LED) light irradiation and Platelet-derived growth factor BB (PDGF-BB) concentration in the wound tissue. The rats were each divided into 4 groups. The wound of each rats was irradiated with a 630 nm LED at a dose of 4 J/cm2.The wound healing were evaluated the wound cell count and the concentration of PDGF-BB. Our results showed that LED treated could accelerate the macrophages, fibroblasts and angiogenesis early 2 days in normal wound rats. And LED treated could also accelerate the macrophages and angiogenesis early 2 days, and the fibroblasts early 4 days in hyperglycemic wound rats. According to the PDGF-BB results, LED treated could accelerate the secretion in normal and hyperglycemic wound rats. In conclusion, we provide evidence that LED irradiation could accelerate the macrophages, fibroblasts and angiogenesis, and then the secretion of PDGF-BB.

並列關鍵字

hyperglycemic rats ； LED ； PDGF-BB

參考文獻

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