以二氧化錫薄膜為基礎研製固態式鈣離子感測器及應用

人體鈣質之濃度係重要健康指標之一，隨生醫感測器快速發展，可達到提供人類簡易、輕便、隨時、隨地並且準確快速得知身體內鈣質濃度狀況，進而減少人類因忙碌等各種因素而疏忽身體各項機能所呈現之警訊。因此適當補充人體所需鈣質並隨時注意身體各項機能之狀況及變化，改善不良生活習慣、定期接受健康檢查改正缺點，才能重拾健康，享有優質生活品質及快樂之人生。本論文以電壓式鈣離子選擇電極為研究方向，藉由固態式二氧化錫/氧化銦錫電極為基礎，並以最佳化成分比例製作鈣離子選擇膜，研製以二氧化錫/氧化銦錫為基礎之固態式鈣離子選擇電極。固態式鈣離子選擇電極之原理係藉由離子選擇薄膜，將待測溶液中之鈣離子進行感測，造成二氧化錫/氧化銦錫電極內之表面電位改變，再藉由電子電路擷取二氧化錫/氧化銦錫電極與參考電極之電壓差，取得待測鈣離子之濃度。依據最佳化成分實驗結果，可得知以二氧化錫/氧化銦錫為基礎之固態式鈣離子選擇電極感測度約29.41 mV/decade，量測極限約1.15x10-6 M，反應時間約7秒，使用壽命約70天，酸鹼量測環境約介於pH 5.0 ~ pH 7.0。本研究亦探討不同金屬離子干擾係數與非理想效應現象，並實際應用於市售鮮奶內鈣離子濃度之量測。依據研究結果，可得知以二氧化錫/氧化銦錫為基礎之固態式鈣離子選擇電極，其優點係製程步驟簡易、成本低、具備良好感測特性及其元件架構可進行微小化。

關鍵字

生醫感測器；固態式二氧化錫/氧化銦錫電極；鈣離子選擇膜；干擾係數；固態式鈣離子選擇電極

並列摘要

Concentration of calcium in human body is one of the key indexes for physical status. With the fast development of biosensors, human being can promptly and easily monitor calcium concentration and other functionality indexes to prevent neglecting form the busy regime. Promptly calcium supplying, regularly physical condition and functionality indexes monitoring, is the key to an enjoyable, quality and happy life. This paper reports a potentiometric solid-state calcium ion-selective electrode (ISE) based on a solid-state SnO2/ITO electrode. Furthermore, three different compositions of calcium ion selective membrane were compared. The calcium ion selective membrane was formed on the sensing window, and the surface potentials of the calcium membrane are changed with different concentration solutions. According to the experimental results, the sensor shows the sensitivity of 29.41mV/decade between 10-6 M and 1.0M calcium concentrations, the low detection limit was about 1.15x10-6M, the response time t95 ( 95% of the steady state signal) was about 7 seconds ,the life-time was about 70 days and stable response was in pH 5.0 ~ pH 7.0 solutions. The result of the experiment was that the high voltage of output was measured in high calcium concentration on packing box of milk. The result shows that the calcium ion-selective electrodes based on SnO2/ITO thin film can be applied in the food. Furthermore, the advantages of the calcium ISE are easy to fabricate, good reproducibility, stability, and low cost for mass production.

並列關鍵字

Biosensor ； solid-state SnO2/ITO electrode ； solid-state calcium ion-selective electrode (ISE) ； selectivity coefficient. ； calcium ion selective membrane

參考文獻

[1] S. Daunert, L. G. Bachas, V. Schauer-Vukasinovic, K. J. Gregory, G. Schrift, and S. Deo, "Calmodulin-mediated reversible immobilization of enzymes," Colloids and Surfaces B: Biointerfaces, vol. 58, pp. 20-27, 2007.

[2] J. Alonso, J. Artigas, and C. Jimenez, "Analysis and identification of several apple varieties using ISFETs sensors," Talanta, vol. 59, pp. 1245-1252, 2003.

[3] A. Bratov, N. Abramova, C. Dominguez, and A. Baldi, "Ion-selective field effect transistor (ISFET)-based calcium ion sensor with photocured polyurethane membrane suitable for ionised calcium determination in milk," Analytica Chimica Acta, vol. 408, pp. 57-64, 2000.

[4] A. Bratov, N. Abramova, and C. Dominguez, "Lowering the detection limit of calcium selective ISFETs with polymeric membranes," Talanta, vol. 62, pp. 91-96, 2004.

[6] D. R. Thevenot, K. Toth, R. A. Durst, and G. S. Wilson, "Electrochemical biosensors: recommended definitions and classification," Biosensors and Bioelectronics, vol. 16, pp. 121-131, 2001.

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以二氧化錫薄膜為基礎研製固態式鈣離子感測器及應用

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