常壓噴射電漿製備電漿活化水及其應用研究

Plasma-activated Water Prepared by Atmospheric Pressure Plasma Jet and Its Applications

張舒涵(Shu-Han Chang)

指導教授 ： 魏大欽

中原大學/工學院/化學工程研究所/碩士(2021年)

https://doi.org/10.6840/cycu202100624

摘要

近年來，電漿活化水在生物醫學方面應用極為廣泛，例如：癌細胞處理、傷口癒合和殺菌。本研究使用自組之常壓電漿束，以氬氣為主要氣體並混合不同的反應氣體(空氣、氮氣、氧氣)製備電漿活化水，檢測不同條件下產生的電漿活化水水中化合物濃度變化，並探討化合物濃度與氣相物種相對濃度的關聯。實驗結果顯示，電漿活化水中含有硝酸、亞硝酸、過氧化氫與臭氧。電漿活化水的酸度和過氧化氫之濃度以氬氣電漿為最高，主要受不同進料氣體產生之電漿強度所影響，電漿強度越大，可在水中產生更多氫離子和羥基，使得pH值更低，並因羥基的覆合作用形成更多過氧化氫。而當進料氬氣加入1%空氣時，水中的亞硝酸根濃度明顯高於其他進料氣體組合，由電漿光譜中發現，亞硝酸根濃度與氣相之一氧化氮相對濃度有關。硝酸方面，在低氣體流量時，以純氬氣電漿最高，因高能量電子可撞擊水面導致較多的NO或NO2在水面上形成，並溶於水產生硝酸鹽，然而在調高氣體流量後，以進料氬氣加入1%空氣時，水中的硝酸濃度最高。本研究也以模擬的化學溶液比較其與電漿活化水對轉醣鏈球菌、大腸桿菌的殺菌效果，發現在pH值2.5時，亞硝酸根不論與過氧化氫亦或是硝酸組合，其對大腸桿菌的滅活能力均可使菌落數低於檢測極限；而轉醣鏈球菌的殺菌實驗可以看出，即使以亞硝酸根、硝酸和過氧化氫組合的化學溶液殺菌，其滅菌的效果依然低於電漿活化水，間接證明，除了電漿活化水中長壽命物質濃度影響了殺菌效果，在電漿活化水中無法量測之短壽命活性氮氧物種在殺菌中也同樣扮演著重要角色。

關鍵字

電漿活化水 ； 殺菌 ； 模擬溶液

並列摘要

Plasma-activated water has been widely applied to biomedical field such as cancer cell treatment, wound healing, and sterilization. In particular, plasma-activated water(PAW) has great potential and high effectiveness on sterilization and bacteria inactivation. This study focuses on activating water with Atmospheric pressure plasma jet (APPJ), using argon as the main gas and nitrogen, air, oxygen as additive gases. The pH value and the concentrations of hydrogen peroxide were found to correlate with plasma intensity under different working gas. If the intensity of plasma enhances, more hydrogen ions and hydroxyl groups can be generated in water which results in the decrease of pH value and the increase of hydrogen peroxide formation. When 1% air was added to argon, the concentrations of nitrite is the highest, and the nitrite concentration correlated with NO relative concentration in the gas phase. Nitrate has the highest concentration when the working gas is argon under low flow rate condition. Plasma jet in water surface cause more NO2 dissolve in water and form nitrate. However, in high flowrate conditions, The concentration of nitrate is highest when 1% air was added to argon. This study also compared the bacteria inactivation between PAW and simulating chemical liquid with Escherichia coli and Streptococcus mutans. Whether nitrite is combined with nitrate or hydrogen peroxide, the simulating chemical liquid can inactivate E.coli below the detection limit. Even if nitrite, nitrate, and hydrogen peroxide are used in the chemical solution, the sterilization effect is still worse than PAW to S. mutans. The results showed that not only the long-lived substances in PAW affected the sterilization but the short-lived substances in PAW which is unmeasurable also played an important role in the sterilization.

並列關鍵字

Plasma-activated water ； Sterilization ； Simulating liquid

參考文獻

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