以MBR處理染整廢水及脫色菌種分析

The Study of Dyeing Wastewater Treatment and Analysis of Decolorization Bacterial by Using Membrane Bioreactor

鄧竣輿(Chun-Yu Teng)

指導教授 ： 游勝傑

中原大學/工學院/土木工程學系/碩士(2007年)

https://doi.org/10.6840/cycu200700445

摘要

近年來國內受水資源開發的限制，工業用水將面臨水資源不足的問題，經濟部水資源局積極推動工業用水回收再利用技術，並對染整業於妥善處理技術及資源回收提出工作重點，希望染整業能達到永續環保發展。故本研究應用薄膜技術，使用厭氧-好氧薄膜生物反應槽（Anaerobic-aerobic membrane bioreactor, AOMBR）及RO膜組(AOMBR/RO)處理人工合成之模擬染整廢水於回收用水可行性分析，並針對厭氧脫色槽之脫色菌種進行分析及鑑定。
本研究第一部份為使用厭氧-好氧薄膜生物反應槽（Anaerobic-aerobic membrane bioreactor, AOMBR）及RO膜組(AOMBR/RO)處理人工合成之模擬染整廢水於回收用水可行性分析。MF膜組分別以M膜、N膜、K膜及C膜操作分析出流水水質，結果得到M膜出流水之COD、BOD、色度及SS去除率各別為97.8%、 98.8%、80.5%及100%，N膜出流水之COD、BOD、色度及SS去除率各別為96.9%、 97.8%、81.9%及100%，K膜出流水之COD、BOD、色度及SS去除率各別為98%、 99.1%、85.3%及100%，而C膜出流水之COD、BOD、色度及SS去除率各別為96.7%、 99 %、84.7%及100%；皆可輕易滿足放流水標準。而在水回收再利用分析結果則皆建議用於沖廁、景觀、澆灌等不與人體接觸及冷卻用水。
各別MF膜組連接RO膜組之出流水以色度的去除為其重點，M/RO、N/RO、K/RO及C/RO膜組出流水之色度去除率分別為99.4%、99.1%、98.6%及99.1%，其水體皆已呈透明無色。而在水回收再利用分析結果同樣建議可用於沖廁、景觀及澆灌等不與人體接觸及冷卻用水。
研究第二部份為厭氧脫色槽中之厭氧脫色菌種之分析，初步脫色實驗結果為6小時內脫色完畢者有5株，定義為高脫色菌株；6~36小時內脫色完畢者有20株，定義為中脫色菌株；而在6~36小時內脫色率大於70%者有25株，定義為低脫色菌株。
高脫色菌經分子生物方式鑑定其菌種為Lactococcus lactis subsp.(乳酸乳球) 菌種，由不提供碳、氮源及不同染料之脫色實驗得知此菌株需大量碳、氮源才能展現高脫色能力且對不同偶氮染料具有高適用性。中脫色菌鑑結果有7株為Lactobacillus(乳酸桿菌)屬而2株為Lactococcus(乳酸球菌)屬。低脫色菌中之AN35鑑定結果為Leuconostoc mesenteroides(腸膜明串念珠)菌種。本研究菌株均為乳酸菌科下之微生物，其親緣相近，故可能具有相似的生化特性、對於染料具有降解能力。但目前仍無法確實指出菌株具有偶氮還原能力。

關鍵字

水回收再利用 ； Reactive Black 5 ； 薄膜生物 ； 脫色菌 ； 染整廢水

並列摘要

Restricted by development of water resources at home in recent years, the water for industrial use will face the problem that the water resource is insufficient, the Water Resource Office activelies promotes the industrial water of reuse technology, and retrieve and put forward the focal point of work in appropriate treatment technology and resource in dyeing the weaving industry, hope to dye the weaving industry to reach and continue environmental protection to develop forever. So this research uses the technology of the membrane, use Anaerobic-aerobic membrane bioreactor (AOMBR), and RO membrane (AOMBR/RO) processes treating the synthesis textile dyeing wastewater on retrieve water feasibility analyse, and analysis and identify of decolourization bacterial of the anaerobic bioreactor.
This first part of research reflects the anaerobic-aerobic membrane bioreactor (AOMBR), and RO membrane (AOMBR/RO) processes treating the synthesis textile dyeing wastewater on retrieve water feasibility analyse. MF Membrane operates and analyses the effluent with M membrane , N membrane , K membrane and C membrane separately, the remove efficiency of COD, BOD, True color and SS is 97.8% , 98.8% , 80.5% and 100% specifically with M membrane effluent, the remove efficiency of COD, BOD, True color and SS is 96.9% , 97.8% , 81.9% and 100% specifically with N membrane effluent, the remove efficiency of COD, BOD, True color and SS is 98% , 99.1% , 85.3% and 100% specifically with K membrane effluent, and the remove efficiency of COD, BOD, True color and SS is 96.7% , 99% , 84.7% and 100% specifically with C membrane effluent ; Can all meet the Taiwan EPA effluent criteria easily. Water recycle analysis result to all propose for the water not contacted with the human body of washing the lavatory, view, irrigator and cooling.
The focal point of MF connection RO membrane processes effluent is the color remove. The remove efficiency of true color is 99.4% , 99.1% , 98.6% and 99.1% respectively with M/RO, N/RO, K/RO and C/RO membrane processes, its effluent has all already been transparent and colourless. Water recycle analysis result to all propose for the water not contacted with the human body of washing the lavatory, view, irrigator and cooling.
The second part of research is analysis of the decolorization bacterial by anaerobic bioreactor, decolour experimental result: To decolour complete for 6 hour have 5 colonies, define for being high decolor bacterial; to decolour complete for 6~36 hour have 20 colonies, define for being medium decolor bacterial; And the person who greater thans 70% of decolouring rate have 25 colonies within 36 hours, define for being low decolor bacterial.
The high decolor bacterial have determined that were Lactococcus lactis subsp. by the molecule biological, the experiment of no offered the carbon and nitrogen, learn that this bacterial strain needs a large number of carbons, nitrogen sources to represent the high ability of decolouring and have high suitability to different azo dyes. The medium decolor bacterial have determined that were Lactobacillus for 7 colonies and 2 colonies belong to Lactococcus. AN35 of low decolor bacterial has determined of Leuconostoc mesenteroides. This research bacterial strain is microorganism under lactic acid bacterial, its blood reason is close , so may have similar biochemical characteristics, have ability of degrading to the dyestuffs. But still unable to really point out that the bacterial strain has azoreduction ability at present.

並列關鍵字

Reactive black 5 ； Dyeing wastewater ； MBR ； Water recycle ； Decolor bacterial

參考文獻

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