- 學位論文
利用電紡絲玻尿酸及其衍生物之奈米纖維改質矽水膠薄膜之研究
Surface Modification of Electrospun Hyaluronic Acid and Its Derivatives Nanofiber on Silicone Hydrogel Membrane
摘要
摘要 長期配戴型矽水膠鏡片具有高透氧特性，但表面為疏水性質，長時間配戴會造成眼球不舒適。本研究目的是透過矽水膠成分與玻尿酸的表面改質改善矽水膠鏡片表面疏水性問題。本研究分成三部分，第一部分測定不同矽含量之矽水膠薄膜的物理性質，第二部分利用甲基丙烯酸環氧丙酯－玻尿酸溶液進行矽水膠表面物理吸附，第三部分利用甲基丙烯酸環氧丙酯－玻尿酸－聚乙烯吡咯烷酮奈米纖維，使用臭氧處理進行表面化學改質。第一部分的結果發現矽水膠中三(三甲基矽氧基)甲基丙烯醯氧丙基矽烷 (TRIS) 含量由 30 vol % 提升至50 vol % 時含水量由 35 % 減少至 28 %，每增加 10 vol % TRIS 並降低 10 vol % 甲基丙烯酸羥丙酯時，含水量則減少 3.5 %。矽水膠機械強度由 0.8 MPa 增加至 4.4 MPa，可見光透光率為 80-95 %，表面接觸角為 92-103 o。第二部分玻尿酸與聚乙烯吡咯烷酮進行物理改質測定電紡絲奈米纖維在 25 oC、50 oC 下進行掃描式電子顯微鏡觀察發現，不同濃度下玻尿酸－聚乙烯吡咯烷酮在 25 oC 纖維直徑分佈為 100-680 nm，50 oC 為 140-770 nm，改質後矽水膠薄膜接觸角為 62-77 o。第三部分矽水膠薄膜化學改質後接觸角為 59-77 o。核磁共振光譜儀測得甲基丙烯酸環氧丙酯－玻尿酸碳碳雙鍵上質子訊號為 5.6 ppm 及 6.1 ppm，合成接枝率為 25.6 %。本研究證明物理性吸附及化學性改質皆能夠將矽水膠薄膜表面調整成為親水性表面。
並列摘要
Abstract Silicone hydrogel contact lenses have high oxygen transmissibility that makes them suitable for extended wear, but their hydrophobic surfaces cause discomfort to the cornea during extended wear. This study aims to adjust the hydrophobic characteristics of the silicone hydrogel by combining siloxane-containing monomers of (trimethyl-siloxy)silyl-propyl methacrylate (TRIS) with hydrophilic monomers, such as 2-hydroxyethyl methacrylate and N-vinyl-2-pyrrolidone. A crosslinking agent and a photoinitiator were also used to fabricate the silicone membrane. Hyaluronic acid (HA) was mixed with polyvinyl pyrrolidone (PVP) to form fibrous membranes using the electrospinning technique. Glycidyl methacrylate-hyaluronic acid (GMHA) was successfully synthesized. Proton nuclear magnetic resonance spectrra showed that the GMHA proton signals were present at 5.6 and 6.1 ppm, representing the vinyl functional group. A solution of GMHA with PVP was used to form fibers by electrospinning. When TRIS was increased by 30-50 vol%, the water content of TRIS-based silicone hydrogel decreased from 35 to 28%. The elastic modulus increased from 0.8 MPa to 4.4 MPa after treatment. The transmittance of the silicone membrane was about 80-95%T. The contact angle at the surface of the silicone membrane was about 92-103o. Scanning electron microscopy analysis showed that the HA-PVP fiber diameters were in the range of 100-680 nm at 25 oC and in the range of 140-770 nm at 50 oC. When silicone membranes were coated with HA-PVP fibers using a physical adsorption procedure, the contact angle decreased from 62 o to77o. In contrast, chemical modification of silicon membranes through ozone treatment and coating with electrospun GMHA-PVP yielded contact angles of 59-77o. Therefore, the techniques presented here have successfully improved the hydrophilic characteristics of a silicon hydrogel membrane.