運用影像分析技術探討單一細胞胞吞磁性奈米粒子之研究

Study of Magnetic Nanoparticles’ Endocytosis in a Single Cell Level with Image Analysis

謝菲妮(Jefunnie Matahum)

指導教授 ： 葛宗融 博士

中原大學/工學院/生物醫學材料與科技國際碩士學位學程/碩士(2017年)

https://doi.org/10.6840/cycu201700892

摘要

磁性米顆粒(MNP)是生物醫學應用廣泛研究的材料之一。 用於量化納米顆粒細胞攝取的分析方法已經很好地建立，然而，單細胞水準的圖像分析仍需要更多的描述。 本文提出了一種替代的非破壞性量化方法,使用圖像分析來量化單個細胞水準的磁性納米顆粒攝取。 據推測，MNP的細胞攝取可以被視為碎形物件來描述細胞內材料的複雜性/形狀。 孵化時間的增加與MNP碎形維度(p <0.05)的增加相關，而MNP濃度的增加與細胞攝取的MNP面積(p <0.05)增加相關。 進行磁電泳實驗以計算單個細胞中的MNP的數量。 結果顯示， 在外部磁場下，當孵育時間從6小時增加到24小時，細胞吸收MNP的量增加為3倍。 而沒有外部磁場暴露的量則增加2.5倍。 這意味著施加額外外部磁場時，細胞具有更高的攝取效率。計算相同重量的Fe奈米粒子數量，並使用ICP-MS分析方法定量細胞，兩者呈現的濃度幾乎相同。 基於以上結果，磁電泳和碎形分析是具有潛能成為MNP在單細胞水準量化的替代品。

關鍵字

影像分析 ； 碎形维度 ； 磁電泳

並列摘要

Magnetic nanoparticles (MNPs) are one of the extensively researched material for biomedical applications. Analytical methods for quantification of cell uptake of nanoparticles are already well established, however, image analysis on single cell level still needs more characterization. This paper presents an alternative non-destructive quantification method using image analysis to quantify the uptake of magnetic nanoparticles in a single cell level. It is reasoned that the cellular uptake of MNPs can be viewed as a fractal object to describe the complexity/shape of the material inside the cell. The increase in the time of incubation correlates with the increase in fractal dimension (p<0.05) of MNP while the increase in the concentration of MNP correlates with the increase in area (p<0.05) of MNP uptaken by the cell. Magnetophoresis experiments were also performed to calculate the number of MNPs in a single cell. It is shown that the amount of MNP uptaken by cells under external magnetic field is increased 3 fold when the incubation time is increased from 6 h to 24 h. While the amount is increased 2.5 fold without external magnetic field exposure. These suggest that the cells will have higher uptake efficiency when simulated with external magnetic field. The equivalent Fe weight of the number of nanoparticle were calculated and rendered almost the same concentration with cells quantified using ICP-MS analysis. Based on the results, magnetophoresis and fractal analysis are good candidate as alternative for quantification of MNP in single cell level.

並列關鍵字

Image analysis ； fractal dimension ； magnetophoresis

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