- 學位論文
聚苯胺/羧酸化石墨烯摻合體於過氧化氫與葡萄糖檢測器之應用
Polyaniline/carboxylated Graphene for sensing the hydrogen peroxide and glucose
摘要
本研究是以化學法製備出石墨烯氧化物 (graphene oxide)，並以紫外光-可見光-近紅外光 (UV-Vis near IR) 光譜分析及熱重分析儀 (Thermogravimetric Analyzer，TGA)、X射線電子能譜儀 (X-ray photoelectronSpectrometer，XPS) 來探討石墨烯氧化物的結構物性。並以穿透式電子顯微鏡 (Transmission Electron Micrsope，TEM) 觀察其表面型態。並利用不同濃度比例之聚苯胺 (polyaniline，PAn) 與石墨烯衍生物摻混，其摻合體修飾之金電極作為過氧化氫與電化學檢測器之工作電極，探討無酵素條件下偵測溶液中過氧化氫最佳化條件。此外由紫外光-可見光-近紅外光 (UV-Vis near IR)、FT-IR和XPS分析graphene oxide/PAn blend 的摻雜程度依序為9.5/ 0.5、9/1、8/2、7/3、6/4。當以摻合體比例為9/1在無酵素最佳條件下偵測H2O2，電流靈敏度為最高值為455 µA/Mm-cm2 (n=5)，偵測線性範圍為0.025 ~ 12.5 mM，最小偵測濃度為0.015 mM (S/N=3)。當以摻合體比例為7/3最佳條件下偵測葡萄糖，其電流靈敏度為27.08µA/mM-cm2，可偵測線性範圍為1~10 mM，最小偵測濃度為0.331 mM (S/N=3)。
並列摘要
The preparation, characterization, and sensor application of the long- and short-chain graphene oxide derivatives prepared by chemical method were investigated in this study. Their structures were analyzed by UV-Vis-near IR spectroscopy, thermal gravity analyzer (TGA), and X-ray photoelectron spectroscopy (XPS), and transmission electron microscope (TEM), respectively. These graphene oxide derivatives were blend with polyaniline (PAn) and the composites were used to coat on the surface of Au as a working electrode for enzyme-free H2O2 and electrochemical sensors. The optimal conditions of the detection of H2O2 were proposed. Meanwhile, the doping levels of graphene oxide/PAn with the concentra- tion ratios of 9.5/0.5, 9/1, 8/2, 7/3, and 6/4 were analyzed by UV-Vis near IR, FT-IR, and XPS. At the optimal molar ratio of 9/1, the linear range of enzyme-free H2O2 sensor was 0.025~12.5 mM with a sensitivity of 455 µA/mM-cm2 (n=5). The limit of detection concentration was 0.015 mM (S/N=3). For glucose detection, the linear range was 1~10 mM with a sensitivity of 27.08µA/mM-cm2 (n=5) at the optimal molar ratio of 7/3. The limit of detection concentration was 0.331 mM (S/N = 3).