分析金奈米粒子與淚液蛋白質之相互作用

Analysis of the interaction between gold nanoparticles and tear proteins

蔡政家(Jheng-Jia Tsai)

指導教授 ： 林政鞍

中原大學/工學院/生物醫學工程研究所/碩士(2019年)

https://doi.org/10.6840/cycu201900358

摘要

眼睛是人體重要的器官之一，長時間暴露在外在環境中，卻能夠抵抗微生物及微粒的感染，主要仰賴其防禦系統中的眨眼反射、淚液、角膜所給予的保護，而淚液中的蛋白質為重要的抗菌成分，且能維持眼睛的功能。近年來因金奈米粒子其獨特的光學特性以及其高生物相容性，並且能透過金硫鍵接枝不同的官能化基團，而有不同的生醫應用，但目前沒有研究針對金奈米粒子進入到淚液中會受到那些蛋白質的吸附及影響，因此本研究透過SDS-PAGE鑑定金奈米粒子表面吸附的蛋白質，對於金奈米粒子的穩定性又有何影響，再搭配標準蛋白質進行實驗，利用吸收光譜儀及水平電泳進行分析，以提供未來金奈米粒子應用於淚液檢測之參考依據。在淚液與金奈米粒子的反應中，乳鐵蛋白及溶菌酶為主要吸附之蛋白，而且兩種蛋白間有競爭現象。透過乳鐵蛋白及溶菌酶混合液，針對金奈米粒子穩定性進行分析，發現兩者的濃度比值，可在水平電泳中初步判斷金奈米粒子的穩定性，最後本研究透過加入固定濃度的溶菌酶作為引發劑，並以金奈米粒子作為感測器，將人體淚液進行序列稀釋，搭配水平電泳，便能夠針對人體淚液進行健康度評估，期望此技術未來能應用於臨床診斷，以利醫師能更準確的判斷及提供金奈米粒子用於眼科醫學領域的應用。

關鍵字

蛋白質暈狀體 淚液 乳鐵蛋白 金奈米粒子

並列摘要

The eye is one of the important organs of the human body. It is exposed to the external environment for a long time, but it can resist the infection of microorganisms and particles. It depends on the defense system, which is corneal reflex, tears and cornea. The proteins in the tears is an important antibacterial ingredient that maintains the function of the eyes In recent years, there have been different biomedical applications due to the optical properties, high biocompatibility of gold nanoparticles, and it can use Au-S bonds to conjugate different functional groups, but there is no research about the Gold nanoparticles into the tears is affected by the adsorption and influence of tears proteins. Experiment of standard protein, using absorption spectroscopy and gel electrophoresis to provide reference or application of gold nanoparticles in tear detection. In the reaction of tears and gold nanoparticles, lactoferrin and lysozyme are the main adsorbed proteins, and there is competition between the two proteins. The lactoferrin and lysozyme mixture is analyzed for the stability of the gold nanoparticles, and the stability of the gold nanoparticles can be preliminarily determined in the gel electrophoresis according to the concentration ratio of the two proteins. In this study, by adding a fixed concentration of lysozyme as an initiator, and using gold nanoparticles as a sensor, the human tears were serially diluted, and gel electrophoresis was used to evaluate the health of human tears. It is expected that this technology can be applied to clinical diagnosis in the future.

並列關鍵字

Protein Corona Tear Lactoferrin Gold Nanoparticle

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