以奈米金粒子結合抗體檢測特定病菌抗 藥性之生物晶片

Development of a Biochip using Antibody-Covered Nano-Gold Particles to Detect Antibiotics Resistance of Specific Bacteria

余華偉(Hua-Wei Yu)

指導教授 ： 黃榮堂

國立臺北科技大學/機電學院/機電整合研究所/碩士(2008年)

摘要

盡早檢測出病菌有無抗藥性不但有助於醫師選擇適當的抗生素，也可以避免抗生素濫用的情形。本研究之目的為發展簡單、快速之檢測方式好取代早期檢測細菌及其抗藥性之方式，利用微機電製程技術製作用於實驗之微粒檢測的裝置晶片。此實驗大致分為三個部份：微電極的製作、病菌收集計數量測、檢測病菌抗藥性可能性。其檢測方式主要是利用介電泳力的作用使溶液中欲檢測的病菌能夠收集於微電極的作用範圍內，提高計數的正確性及準確性。同時，本文也對奈米金粒子的作用及在不同形狀電極下加入奈米金抗體混合病菌檢測之情形加以探討。
本實驗室利用玻璃基材及CD 光碟片製作出不同形狀的梳狀電極後，測量其結果；結果顯示平行電極最有利於奈米金粒子實驗之進行。最後，再以平行電極測量病菌，探討其有無抗藥性。

關鍵字

微機電製程技術 ； 介電泳力 ； 奈米金粒子 ； 抗藥性檢測

並列摘要

Detection of bacteria’s antibiotics resistance at an early stage not only helps doctors in choosing the appropriate antibiotics, but also prevents antibiotic abuses. The purpose of this research is to develop a simple, efficient method for testing bacteria and detecting bacteria’s antibiotics resistance, which can replace the traditional method. This research uses MEMS technology to construct the particle counting chip which will be used in the experiment. The experiment can be separated into three distinct parts: designing electrodes, cells collect and measure, and the detection of bacteria’s antibiotics resistance. The detection method primarily uses the effect of DEP force to ensure that the bacteria in the solution can be collected within the range of electrodes, increasing the validity and accuracy of the count. Also, the text explores the effect of gold nano-particles and the bacteria’s reaction to the addition of gold nano-particles
antibody under different shapes of electrodes.
The research uses glass substrate and CD disc to produce different comb-like electrodes for measurement; results show that Parallel electrodes is most favorable for
the experiment. Lastly, Parallel electrodes is used for testing the bacteria, indicating whether if the bacteria are antibiotics-resistant.

並列關鍵字

MENS ； Dielectrophoresis(DEP) ； nano-gold ； measurement of bacteria’s antibiotics resistance

參考文獻

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