- 學位論文
高通量顯微影像系統之開發
Development of a High-throughput Microscopy Image System
摘要
本研究結合影像處理、機械視覺、自動化控制與平台設計等，來設計一套擁有高通量顯微鏡之初步功能的設備，對於需要以顯微鏡取得大量研究資料的實驗，不再受限於昂貴的設備。只需要將設備直接搭配在現有的顯微鏡設備上，配合自動對焦、目標跟蹤等演算法，使顯微鏡擁有可以大量，並且長時間的取得目標的影像資訊，並且利用失焦重建與三維輪廓的建立來幫助在目標的觀測。本研究經過實驗驗證，針對使用PCR-96孔培養皿的目標物進行觀察，最高可同時觀察72(9*8)個樣本，當針對單一目標進行影像追蹤時，可以長時間使目標維持在觀察視窗內。目前所設計的平台最大行程為105mm ，並擁有±1 µm/105mm的精度，已可滿足在一般顯微鏡上的使用需求。
並列摘要
The study is integration of image processing, machine Vision, automation control and platform design, The project deals with the building of a universal functioning piece of equipment with the application of high-throughput microscopy that is in comparison with the microscope we currently use now, For data acquisition needs a microscope to obtain a lot of image information, we do not need to purchase expensive equipments, which can make the microscope operate with high throughput and long operation time. In addition, the built-in functions in auto-focusing, image tracking, image registration, and three-dimensional surface profile reconstruction are extremely helpful to the observations of targets. Experiments were carried out with the observation of target cultured in a PCR-96-hole culture dish. The maximum observed targets are 72 samples simultaneously. When tracking a single target, the target can be maintained in the observation window as long as wish. The current maximum stroke of the platform is 105mm, and a precision of ± 1 μm/105mm can be reached. This fulfills the needs of a microscope for general applications.