大氣電漿輔助誘導接枝改質聚醚醚酮表面固定生醫單體活性之研究

Atmospheric Plasma Assist Induced Graft to Surface Modification of Polyetheretherketone for Study of Immobilized Biomolecules

吳信儀(Yi-Xin Wu)

指導教授 ： 王明誠

中原大學/工學院/生物醫學工程研究所/碩士(2022年)

https://doi.org/10.6840/cycu202200029

摘要

根據衛生福利部的調查結果顯示，近年來台灣18歲以上成年人中，患有不等程度的牙周病，嚴重的話牙齒會移位或鬆動導致缺牙現象，顯示目前對牙科植入材料的需求大大提升。植牙是缺牙治療最常使用方式，聚醚醚酮為最接近人體骨骼的材料，具有良好的機械性能、化學穩定性等許多優點。因此已開發為金屬和陶瓷材料植入物的替代方案，得以改善金屬材料會引起的過敏反應以及應力遮蔽效應等缺點，而陶瓷材料的低延展性和脆性會限制陶瓷的使用。但聚醚醚酮其生物活性不佳，需利用表面改質接枝來提升其生物活性，而大氣電漿廣泛應用在表面改質，故本研究利用大氣電漿輔助誘導接枝對聚醚醚酮進行表面處理來產生官能基增強表面活性，本研究透過電漿設備利用氣壓0.1MPa功率1000W、電漿噴頭距離15 mm、時間設定為10秒，將聚醚醚酮置入電漿機台進行電漿輔助接枝表面處理，使表面產生氧化基和過氧化基，再使用1mL 3%、10%的丙烯酸單體裝入噴筆均勻的噴在試片的表面20秒，再進行1次電漿輔助表面處理10秒，讓丙烯酸單體接枝到表面上，完成接枝後將試片以60℃水浴超音波震盪30分鐘，把沒有接枝上的AAC 清洗掉，再進行誘導接枝固定BMP-2。利用FTIR發現接枝濃度10%丙烯酸效果較佳，所以選定接枝10%丙烯酸濃度來進行誘導接枝固定BMP-2增加活性。在聚醚醚酮的表面，從結果可以看出吸收峰 (Plasma assisted graft 10% AAC)上 C=O 鍵的位置為1743 cm^(-1)、O-H彎曲範圍1485 cm^(-1)和925 cm^(-1)之間。誘導接枝固定BMP-2 (Plasma assisted graft 10% AAC+EDC/NHS+BMP-2)，從FRIR結果可以看出C=O鍵的吸收峰位置為1743 cm^(-1)改變為1557 cm^(-1)，EDC/NHS上的N-H鍵的吸收峰位置為1680 cm^(-1)、C-N 鍵吸收峰位置為1672 cm^(-1)、BMP-2 吸收峰位置為1664 cm^(-1)，確定連接了EDC/NHS和誘導接枝固定BMP-2。且利用FE-SEM接枝不同放大倍率的表面形態，表面經過電漿處理接枝比未處理電漿的表面還要光滑，顯示表面的確有不同於原始的物質在上面，但兩者接枝濃度不同的狀況下可以發現PEEK(電漿輔助接枝 10S/AAc 10%)的表面比PEEK(電漿輔助接枝 10S/AAc 3%)還要光滑。此目的為改善牙科植入物的缺點，藉此提升植入物的骨整合效應。

關鍵字

大氣電漿 ； 聚醚醚酮 ； 表面改質 ； 表面分析

並列摘要

According to the survey results of the Ministry of Health and Welfare, in recent years, adults over the age of 18 in Taiwan suffer from periodontal disease of varying degrees. In severe cases, the teeth will be displaced or loosened resulting in missing teeth. Demand has greatly increased. Dental implants are the most commonly used method for the treatment of missing teeth. Polyetheretherketone is the material closest to human bones and has many advantages such as good mechanical properties and chemical stability. Therefore, it has been developed as an alternative to metal and ceramic material implants, which can improve the shortcomings of allergic reactions and stress shielding effects caused by metal materials, while the low ductility and brittleness of ceramic materials can limit the use of ceramic materials. However, polyether ether ketone has poor biological activity, and surface modification grafting is needed to improve its biological activity, and atmospheric plasma is widely used in surface modification. The surface treatment of ketone is carried out to generate functional groups to enhance the surface activity. In this study, the polyetheretherketone was placed in the plasma machine with the air pressure of 0.1MPa and the power of 1000W, the distance of the plasma nozzle was 15 mm, and the time was set to 10 seconds. Plasma-assisted grafting surface treatment, to generate oxide groups and peroxide groups on the surface, and then use 1mL of 3%, 10% acrylic monomers to put into the airbrush and spray evenly on the surface of the test piece for 20 seconds, and then conduct 1 electroporation. Slurry-assisted surface treatment for 10 seconds to allow the acrylic monomer to be grafted onto the surface. After the grafting was completed, the test piece was ultrasonically oscillated in a water bath at 60°C for 30 minutes, and the AAC that was not grafted was washed away, and then induced grafting was performed. BMP-2. Using FTIR, it was found that the grafting concentration of 10% acrylic acid had better effect, so the grafting concentration of 10% acrylic acid was selected to induce the grafting and immobilization of BMP-2 to increase the activity. On the surface of PEEK, it can be seen from the results that the position of the C=O bond on the absorption peak (Plasma assisted graft 10% AAC) is 1743 cm^(-1), the bending range of OH is 1485 cm^(-1) and Between 925 cm^(-1). Induced graft fixation of BMP-2 (Plasma assisted graft 10% AAC+EDC/NHS+BMP-2), from the FRIR results, it can be seen that the absorption peak position of C=O bond is 1743 cm^(-1) changed to 1557 cm^(-1), the absorption peak position of NH bond on EDC/NHS is 1680 cm^(-1), the absorption peak position of CN bond is 1672 cm^(-1), and the absorption peak position of BMP-2 is 1664 cm^(-1), it was confirmed that EDC/NHS was attached and BMP-2 was immobilized by induced grafting. And using FE-SEM to graft the surface morphology of different magnifications, the surface grafted by plasma treatment is smoother than the surface of untreated plasma, showing that the surface is indeed different from the original material on it, but the two graft concentrations It can be found that the surface of PEEK (plasma-assisted grafting 10S/AAc 10%) is smoother than PEEK (plasma-assisted grafting 10S/AAc 3%) under different conditions. The purpose is to ameliorate the disadvantages of dental implants, thereby enhancing the osseointegration effect of the implant.

並列關鍵字

atmospheric plasma ； polyetheretherketone ； surface modification ； surface analysis

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