以接枝環氧化磺基甜菜鹼共聚物對聚對苯二甲酸乙二酯薄膜進行功能化以製備抗沾黏生物相容性材料

設計抵抗生物分子沾黏之薄膜預防非特異性蛋白吸附、細胞黏貼、細菌貼附，是對於傷口癒合至關重要的研究。本研究對聚對苯二甲酸乙二酯薄膜(PET)表面進行接枝環氧化磺基甜菜鹼共聚物(poly(GMA-co-SBMA))，接枝完成後改變薄膜表面之化學結構及親疏水特性和水合之程度。使用動態水接觸角量測儀、X射線光電子能譜儀去鑑定接枝後薄膜表面之物理結構與化學性質；而接枝後的薄膜(PET-g-PGMA-co-SBMA)的抵抗生物分子沾黏特性透過人體血漿纖維蛋白原(Fibrinogen, FN)、螢光大腸桿菌(Escherichia coli, E.coli)、人類腫瘤細胞(HT-1080)的貼附與血液相容性等實驗進行測試，證明改質薄膜確實擁有良好抵抗生物分子沾黏之特性。本研究藉由大鼠動物模型進行傷口敷料測試，觀察傷口在接枝環氧化磺基甜菜鹼共聚物之薄膜(PET-g-PGMA-co-SBMA)覆蓋的傷口癒合結果。在4天及7天後的表面傷口觀察結果顯示，接枝後的親水雙離子化薄膜提供了傷口良好的癒合環境，傷口在初期恢復速度優於其他市面上的商業敷料，如:人工皮、紗布、水膠，證明薄膜上接枝環氧化磺基甜菜鹼共聚物後，具有良好抵抗生物分子沾黏的特性，有助於皮膚組織的快速再生並驗證此材料有作為生物惰性材料與人體植入物的潛力。

關鍵字

甜菜鹼聚合物、抗生物沾黏、聚對苯二甲酸乙二酯、類雙離子、共聚高分子

並列摘要

Design of anti-biofouling membrane to prevent non-specific protein adsorption, cell adhesion, and bacterial attachments is critical to development of advanced wound dressing. In this research, anti-fouling functionalization of poly(ethylene terephthalate) membrane was achieved through coating to epoxylated sulfobetaine copolymers(poly(GMA-co-SBMA). Characterization the surface structure, hydration capacity and hydrophilic property of the membrane were evidenced by dynamic contact angle, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy (FTIR). The anti-biofouling characteristics of modified membrane were demonstrated by fibrinogen protein、Fluorescent Escherichia coli、Human cell culture (HT-1080) growth and blood cell attachment experiments. The results proved that the modified membrane were resistant to the adhesion from a wide range of biomolecules. Furthermore, the potential of the new PET membranes serving as novel wound dressing was evaluated by rat animal model. The healing rate of wound were compared among the ones covered with the modified membrane and commercial patches. The results of surface wounds after 4 days and 7 days showed that the zwitterionic PET membrane provided a good wound healing environment for faster recovery than the artificial skin, gauze, hydrophilic hydrogel could provided. The membrane grafted epoxylated sulfobetaine copolymer were thus proven biocompatible and has the potential as a cost effective implant material.

並列關鍵字

Polybetaine、Antifouling、Poly ethylene terephthalate 、Zwitterionic 、Co-polymer

參考文獻

52. 張雍, 葉., 雙離子性高分子材料界面之血液相容性質. 台灣化工期刊, 2015. 62(1): p. 92-102.

1. Castner, D.G. and B.D. Ratner, Biomedical surface science: Foundations to frontiers. Surface Science, 2002. 500(1-3): p. 28-60.

2. Senaratne, W., L. Andruzzi, and C.K. Ober, Self-assembled monolayers and polymer brushes in biotechnology: Current applications and future perspectives. Biomacromolecules, 2005. 6(5): p. 2427-2448.

3. Amiji M, P.K., Surface modification of polymeric biomaterials with poly(ethylene oxide), albumin, and heparin for reduced thrombogenicity. J Biomater Sci Polym Ed., 1993. 4(3): p. 217-34.

4. Emanuele Ostuni, R.G.C., R. Erik Holmlin, Shuichi Takayama, and and G.M. Whitesides*, A Survey of Structure-Property Relationships of Surfaces that Resist the Adsorption of Protein. Langmuir, 2001. 17: p. 5605-5620.

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以接枝環氧化磺基甜菜鹼共聚物對聚對苯二甲酸乙二酯薄膜進行功能化以製備抗沾黏生物相容性材料

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