製備順向聚己內酯 /聚比咯靜電紡絲及其應用於吸附牛血清白蛋白

隨著科技發展蛋白質體學逐漸變為生物醫學研究重點，因此生物技術和製藥工業投入大量的努力開發有效、快速分離和高純化度蛋白質的方法。本研究的目的是運用一導電靜電紡絲於蛋白質吸附，除了藉由靜電紡絲的高孔隙率及高比表面積，更能夠結合導電高分子的電學特性，提升蛋白質的吸附效率。本研究使用超音波震盪方式氧化聚合聚比咯，以傅立葉轉換紅外光光譜儀驗證官能基，成功聚合聚比咯導電高分子。掃描式電子顯微鏡影像結果顯示使用3000轉速的滾筒式收集方式可以使靜電紡絲的直徑減小且提高順向度。實驗結果顯示聚己內酯紡絲纖維經過表面聚合聚比咯後由高疏水性表面轉變為親水性的表面，其接觸角從132.4±0.83°下降至87.8 ± 0.95°；導電度最高可達到0.147±0.014 S/m，高度順向的導電紡絲能幫助電流在結構中傳遞，可大幅提高材料導電度。此外，將導電紡絲纖維給予5 V電位後進行蛋白質的吸附實驗，與未給電位之對照組相比，蛋白質吸附效率皆明顯上升，且在吸附平衡實驗結果顯示，施加電位可以使飽和吸附量上升；通過重複吸附的方式亦可以有效提升導電紡絲纖維對牛血清白蛋白的吸附量，最高吸附率達到93 %。本研究成功運用導電紡絲高分子的導電特性提升蛋白質吸附量，未來可望在生物醫學的檢測上扮演重要角色。

關鍵字

聚比咯；導電紡絲纖維；蛋白質吸附；牛血清白蛋白

並列摘要

Because of the development of technology, proteomics has become the focus of biomedical research, so the biotechnology and pharmaceutical industry put a lot of effort to develop efficient, rapid separation and high purity for protein purification. The purpose of this study is to apply a conductive electrospun for protein adsorption by their high porosity and high specific surface area. Combined electrical properties of conductive polymers with the electrospun were utilized to enhance the adsorption efficiency of proteins. In this study, we used the oxidative polymerization method to polymerize polypyrrole with sonication. The electrospinning fibers were characterized by Fourier transform infrared spectroscopy. The morphology and fibers size were observed by scanning electron microscope and transmission electron microscope. The results showed that the diameter of the electrospinning fibers can be reduced and become more uniform when the rotary collector is set at higher speed. The highly aligned conductive fiber structure can aid in the current transfer, thus greatly improving its conductivity. The conductivity of fibers is 0.147 ± 0.014 S/m at 3000 rpm. The contact angle of fibers was changed from 132.4±0.83° to 87.8±0.95° after oxidative polymerization which means the fibers became hydrophilic from hydrophobic state. The electrospun fibers were subjected to the protein of bovine serum albumin (BSA) adsorption experiments after potential was applied. The adsorption efficiency was increased compared to the electrospun fibers without the potential group. Adsorption equilibrium experimental results show that the application of potential saturation adsorption capacity was increased. The adsorption capacity of BSA was increased by repeat adsorption method, and the maximum adsorption can reach 93% after repeating three times testing. In this study, we successful using conductive electrospinning fibers to improve protein adsorption behavior which can be a potential candidate for biomedical detection application.

並列關鍵字

Polypyrrole ； Conductive electrospun fibers ； Protein adsorption ； Bovine serum albumin

參考文獻

[1] H. Shirakawa, E. J. Louis, A. G. MacDiarmid, C. K. Chiang, A. J. Heeger, “Synthesis of electrically conducting organic polymers: halogen derivatives of polyacetylene, (CH)x,” Journal of the Chemical Society, Chemical Communications, pp. 578-580, 1977.

[2] C. Conesa, M. Calvo, L. Sanchez, “Recombinant human lactoferrin: A valuable protein for pharmaceutical products and functional foods,” Biotechnology Advances, vol. 28, pp. 831-838, 2010.

[3] B. K. Nfor, P. Verhaert, L. A. M. van der Wielen, J. Hubbuch, M. Ottens, “Rational and systematic protein purification process development: the next generation,” Trends in Biotechnology, vol. 27, pp. 673-679, 2009.

[4] O. Hardick, S. Dods, B. Stevens, D. G. Bracewell, “Nanofiber adsorbents for high productivity downstream processing,” Biotechnology and Bioengineering, vol. 110, pp. 1119-1128, 2013.

[5] S. E. Moulton, J. N. Barisci, A. Bath, R. Stella, G. G. Wallace, “Investigation of protein adsorption and electrochemical behavior at a gold electrode,” Journal of Colloid and Interface Science, vol. 261, pp. 312-319, 2003.

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製備順向聚己內酯 /聚比咯靜電紡絲及其應用於吸附牛血清白蛋白

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