發展磁阻式生物感測系統應用於磁珠偵測

磁性粒子應用於生醫領域已有數十年，透過磁性粒子的高操作性與高結合性可應用於多種生物分子檢測，然而檢測系統化與偵測多樣性是目前研究上鮮少發展的，因此本研究之目的為開發一套以巨磁阻利用磁性粒子作為感測目標達到磁粒子定量之系統，並建立一組標準測試流程，供後續不同感測器與不同粒徑之磁性粒子使用。利用巨磁阻感測器於不同外加磁場下量測磁性粒子，透過LabView呈現感測器於鎖頻放大器與自製訊號擷取裝置之訊號。結果顯示本研究設計之鎖頻放大量測系統中，在垂直磁場100 G、水平磁場2 G、流速0.4 ml/min以及鎖頻參數10 Hz下量測磁性粒子具有最佳的訊號表現，並藉由簡易自製訊號擷取裝置取代昂貴之鎖頻放大器，以簡單操作顯示計數結果完成自動化流程，在相同實驗參數下，最高靈敏度可達10 μg/μl，經由上述驗證結果得知，本研究設計之標準測試流程能有效抓取磁性粒子於感測器中最大訊號表現，並大幅提升自製感測系統取代鎖頻放大量測系統之可行性。

關鍵字

鎖頻放大器；磁阻感測器；磁性粒子感測系統

並列摘要

Application of magnetic particles (MPs) has been applied in biomedical field for many years. There are a lot of advantages through this mediator including highly biocompatibility and multi-diversified in bio-application. However, current techniques for evaluating the quantity of the magnetic labeled sample assays are rare. In this thesis, a homemade integrated detective system with a Wheatstone bridge giant magnetoresistance (GMR) sensor inside microﬂuidic channels was fabricated and used to detect amount of MPs. Quantitative signals will be captured and compared by Lock-In amplifier and homemade device. In this work, MPs have the best signal performance with 100 G of vertical magnetic field and 2 G of horizontal magnetic field applied, 0.4 ml/min flow rate and 10 Hz frequency in Lock-In measure system. Furthermore, homemade signal acquisition device replaces Lock-In amplifier and makes detection automatic. Base on the same parameter, homemade system has shown high detecting sensitivity with 10 μg/μl of MPs amount. In conclusion, this study has built up a standard procedure which is the best performance in this system.

並列關鍵字

Lock-In amplifier ； magnetoresistance sensor ； magnetic beads sensing system

參考文獻

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發展磁阻式生物感測系統應用於磁珠偵測

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