二維影像小鼠胚胎幹細胞螢光蛋白區域之辯識及分化量化之分析

以量化分析技術來辨識細胞分化時之形態及其特徵變化近年廣被使用。我們以胚胎幹細胞共軛焦顯微鏡之螢光影像，分析胚胎幹細胞分化時之時序列之形態及特徵之變化及面積、周長和半徑長度等各項幾何性質，以幫助生物學家量化分析細胞各螢光反應區的時序列形態變化，以有效率引導分化之轉錄因子等培養條件，闡明胚胎幹細胞分化機制來幫助基礎生物學和幹細胞轉譯的研究，以促進再生醫學之實用。本文提出了 2D 共軛焦顯微鏡螢光影像之雜訊去除方式，再就 2D 圖像螢光區域分割，使用了從各個顯微鏡圖像中的分割區域的半徑和中心，再歸類。實驗結果表明，所提出的處理方法可以合理的反應出螢光反應區之 2D 圖像。

關鍵字

共軛焦螢光顯微鏡；二維細胞區域切割；胚胎幹細胞

並列摘要

Quantitative analysis techniques have been widely used in recent years to identify the morphology and characteristic changes of cells during differentiation. We use the fluorescent images of the embryonic stem cell confocal microscope to analyze the changes in the morphology and characteristics of the embryonic stem cells at the time of differentiation, as well as various geometric properties such as area, circumference, and radius. To help biologists quantify and analyze the time-sequence morphological changes of the fluorescent reaction regions of cells, to efficiently guide the differentiation of transcription factors and other culture conditions, to clarify the differentiation mechanism of embryonic stem cells to help the research of basic biology and stem cell translation to promote regeneration The practicality of medicine. This article proposes a noise removal method for the fluorescent image of a 2D confocal microscope, and then divides the fluorescent area of the 2D image, using the radius and center of the divided area from each microscope image, and then classifies it. Experimental results show that the proposed processing method can reasonably reflect the 2D image of the fluorescent reaction zone.