不同芒果品種之樹葉的總抗氧化力與芒果苷及綠原酸含量之研究

A Study on Total Antioxidant Capacity and Mangiferin and Chlorogenic Acid Contents in the Leaves of Different Varieties of Mango

陳彥儒(Yan-Ru Chen)

指導教授 ： 葉華光

中原大學/理學院/化學研究所/碩士(2018年)

https://doi.org/10.6840/cycu201800248

摘要

芒果源自北印度，是台灣常見的水果，營養價值頗高，且其葉子根據文獻顯示，具有芒果苷、類胡蘿蔔素等抗氧化物質。
　　本實驗比較三十種不同品種之芒果葉的抗氧化能力及芒果苷含量。利用傳統萃取的最佳條件萃取後，進行FRAP、DPPH、總多酚、總類黃酮、總水解單寧、總縮合單寧等六項實驗，並以HPLC測定綠原酸及芒果苷之含量。實驗結果顯示三十種芒果葉之抗氧化能力，總單寧含量最多，而綠原酸則不存在芒果葉，且海頓芒果葉在六項測試中，有四種位居前三高，其整體抗氧化物含量較其他品種多。
　　除此之外，DPPH跟FRAP這兩項抗氧化能力測試中，品種間的差異性不高，類黃酮以及總縮合單寧的量較具有明顯之差異。
　　透過本實驗了解到芒果葉的抗氧化成分，故相關產業可以將芒果的廢棄物萃取出其抗氧化成分，再製作成抗氧化產品。

關鍵字

高效液相層析法、芒果苷、台灣、芒果、抗氧化

並列摘要

The mango originated from North India. Approximately 50% of the global mango supply is from India. It is a common fruit in Taiwan. It is rich in sugar, protein, vitamin A, vitamin C, and minerals.
Research shows that mango leaves, stems, pericarps, and seeds are also known to have antioxidant properties. Unfortunately, these parts are usually wasted. Therefore, this experiment hopes to give these wastes a new life. I hope that this experiment of extraction can be used as the development and application towards healthy foods like the mango.
This experiment compares the antioxidant capacity and mangiferin content of thirty different varieties of mango leaves. From here, they are taken from optimum conditions for traditional extraction in which they will undergo six different tests. I will measure the antioxidant capacity from: FRAP, DPPH, total polyphenols, total flavonoids, total hydrolyzed tannins, total condensation Tannins. I will also determine the chlorogenic acid and mangiferin content by HPLC. The results showed that the content of tannins was the highest antioxidant capacity in thirty species of mango leaves, while chlorogenic acid was almost undetectable. Also, in the six tests of Haydn mango leaves, 4 were among the top 3 in terms of their overall antioxidant content compared to the other varieties of mangos.
Through this experiment, the antioxidant components of mango leaves can be known. Therefore, related industries can extract the mango wastes from their antioxidant components and transfer them into antioxidant products for the public.

並列關鍵字

mangiferin, HPLC, mango, antioxidant, Taiwan

參考文獻

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