以電紡絲程序製備雙離子型聚偏二氟乙烯奈米纖維
膜於血球分離之應用

本實驗合成了親水性的雙離子共聚物PS-PEGMA-PSBMA及PS-PEGMA-PSBAA，將其個別進行混摻(Blending)於聚偏二氟乙烯(PVDF)溶液中，製備出具有親水特性的電紡絲薄膜。在調配鑄膜液時，各會添加不同含量的雙子共聚物，分別為1wt%、5wt%、9wt%，並且總固體含量固定在20wt%。藉由調控PVDF及雙離子共聚物之間的比例，測試並比較不同條件下，抗沾黏的效果及生物相容性。本實驗也會從中比較PS-PEGMA-PSBMA及PS-PEGMA-PSBAA對於熱穩定性的能力。從實驗中可以得知，PVDF 11wt% 混PS-PEGMA-PSBAA 9wt%(PV11A9)對於蛋白質、細菌以及血液的抗沾黏能力最佳，且利用滅菌釜對電紡絲薄膜高溫滅菌後，藉由抗菌能力去比較可以得知，PS-PEGMA-SBAA相對於PS-PEGMA-SBMA有更好的熱穩定性。紅血球在檢測醫學上為一個主要的干擾源，本實驗嘗試將雙離子電紡絲薄膜應用於血液過濾上，將血球移除並得到一個無血球的濾液。結果顯示利用10倍稀釋的血液，通過5層疊加且串連6次的PV11A9電紡絲過濾膜，血球移除可達100%，由此證明雙離子電紡絲薄膜具有一定阻擋血球的能力。

關鍵字

雙離子、抗沾黏、血液過濾、電紡絲、聚偏二氟乙烯

並列摘要

Our blood contains tons of biomarkers for the diagnoses of different kinds of diseases but usually these biomarkers could be detected in blood plasma. Blood plasma could be prepared via centrifugation of whole blood which can be time consuming and troublesome. In this study, amphiphilic zwitterionic copolymers were synthesized containing segments of polystyrene (PS), poly(ethylene glycol) methacrylate (PEGMA), and either sulfobetaine methacrylate (SBMA) or sulfobetaine acrylamide (SBAA) for the fabrication of an electrospun polyvinylidene fluoride (PVDF) membranes for the filtration of diluted whole blood. The blended membranes were physically and chemically characterized. Also, to test for their future applications, biofouling tests were also conducted as well as stability after wet thermal sterilization. The results show that PV11A9 has a better antifouling property than the rest, it showed 99.88% reduction in fouling relative to the unmodified PVDF. Also, in general SBAA blended membranes performed better with regards to their thermal stability. PV11A9 membrane were used for the blood filtration tests, because it has a good antifouling property of blood cell and bacteria. A complete blood count (CBC) machine was used to quantify the number of blood cells present in the diluted human whole blood (10X) after the blood filtration process. A cell-free permeate was achieved after going through 30 layers of PV11A9 membranes, each small filter was composed of 5 layers of PV11A9 membranes, that is to say, 6 unit of small filters were stacked in series.

並列關鍵字

Zwitterionic, Antifouling, Blood Filtration, Electrospun, PVDF

參考文獻

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