常壓電漿接枝甲基丙烯酸聚乙二醇酯在聚四氟乙烯 薄膜之抗生物沾黏材料運用與探討

Atmospheric Pressure Plasma Jet Grafting of Poly(Ethylene Glycol) Methacrylate on Poly(TetraFluoroEthylene) Membranes for Anti-biofouling Applications

張家仁(Chia-Jan Chang)

指導教授 ： 魏大欽

中原大學/工學院/化學工程研究所/碩士(2009年)

https://doi.org/10.6840/cycu200900847

摘要

中文摘要 本研究以常壓電漿對聚四氟乙烯同時進行表面活化與接枝的步驟，使 膜材接枝上甲基丙烯酸聚乙二醇酯來達到抗蛋白吸附的效果，探討常壓電 漿參數、單體濃度、單體分子量大小等對於接枝結果的影響，再與低壓電 漿處理後的結果進行比較，利用接枝量、水接觸角、全反射式傅立葉轉換 紅外線光譜儀、掃描式電子顯微鏡、X 射線光電子能譜儀等儀器分析表面 化學與物理結構，與酵素免疫分析法所得的抗纖維蛋白結果進行討論。 研究發現常壓電漿功率150W 時會有最佳的接枝量，在單體濃度10 wt%的情況下，接枝量會隨處理時間增加而有上升的趨勢，但是從化學分析 膜面接枝上的單體，會發現隨時間增加單體官能基被破壞程度越嚴重，進 而影響其抗纖維蛋白的效果，此情形可利用提高單體濃度而獲得改善，當 單體濃度上升至30wt%時，單體之PEG 鏈保有度會明顯提升，抗纖維蛋白 能力也會較10wt%佳，當濃度再提升至50wt%時，雖單體結構保有程度上 類似於30wt%，但接枝量會下降而使抗纖維蛋白能力不佳。 將常壓電漿與低壓電漿處理所得的結果相比，可發現低壓電漿處理後 ，接枝量會比常壓電漿高出兩倍以上，且單體保有程度只略差於30wt%時 常壓電漿的處理結果，但抗纖維蛋白能力卻差了2 倍以上，分析原因為常 壓電漿處理後單體以表面接枝為主，會在表面形成較厚的抗蛋白結構，而 低壓電漿主要以膜孔洞接枝為主，表面單體接枝量較少，因此造成抗蛋白 能力上低壓電漿會較常壓電漿差。 本研究所開發的常壓電漿接枝方法可以快速、乾淨、簡便的製造出抗 纖維蛋白吸附表面，除此之外也可運用在其他單體的接枝方面，是一種極 有潛力的技術。

關鍵字

甲基丙烯酸聚乙二醇酯 ； 常壓電漿 ； 聚四氟乙烯

並列摘要

Abstract
To improve the anti-biofouling property, Poly(tetrafluoroethylene) (PTFE) membranes
were grafted with poly(ethylene glycol) methacrylate (PEGMA) by direct treatment under
atmospheric pressure plasma jet (APPJ). The chemical composition and microstructure of the
surface-modified PTFE membranes were characterized by fourier transform infrared
spectroscopy (FTIR), X-ray photoelectron spectroscopy(XPS), contact angle and scanning
electron microscope (SEM). The protein adsorption on the prepared membranes was
evaluated using the method of enzyme-linked immunosorbent assay (ELISA).
It was found that the grafted amount of the co-polymerized PEGMA and hydrophilicity
on the surface of PTFE membranes can be controlled via different plasma treatment time and
initial monomer concentration. Anti-fouling property is affected not only by PEGMA grafted
amount but also by the PEG chain length in the resulting film. For membrane incubated under
monomer concentration of 30wt%, the grafted PEGMA film can be protected form plasma ion
bombardment by the homo-polymer in the uppermost layer, thus the grafted film preserves
most of the PEGMA’s long PEG side-chain, as evidenced by the large C-O/C=O ratio in C1s
peak of XPS spectra. This PTFE-g-PEGMA surface showed relatively small number of
Fibrinogen adhesion.
At last, a comparative study was made by grafting PEGMA onto ePTFE under low
pressure plasma. The cross sectional view of SEM micrographs show that, while the
PEGMA layer is grafted primarily on the PTFE surface when treated under APPJ, the
PEGMA monomer is mostly grafted inside the pores of the membrane when treated under
low-pressure plasmas, resulting in the relatively higher protein adsorption on the
PTFE-g-PEGMA surface. Protein adsorption results revealed that the PTFE-g-PEGMA
surface prepared under atmospheric-pressure plasma jet exhibited better anti-biofouling
property.

並列關鍵字

Poly(tetrafluoroethylene) (PTFE) ； Poly(ethylene glycol) methacrylate

參考文獻

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