以表面電漿共振技術研究自組裝聚氧化乙烯三團聯共聚物之堆疊構形控制及探討其與人體血漿蛋白 之吸附行為

A systematic SPR study of human plasma protein adsorption behavior on the controlled surface packing of self-assembled poly(ethylene oxide) triblock copolymer surfaces

朱婉菱(Wan-Ling Chu)

指導教授 ： 張雍

中原大學/工學院/化學工程研究所/碩士(2009年)

https://doi.org/10.6840/cycu200900925

摘要

控制具有生物相容性之抗沾黏表面對於醫療需求是重要的環節。於本研究中，我們利用物理吸附方式將具有疏水-親水的三團聯共聚物聚氧乙烯-聚氧丙烯-聚氧乙烯(PEO-PPO-PEO)，吸附於自組裝單層膜的表面CH3-SAM上。研究系統以兩種不同系列的PEO-PPO-PEO來改變基材表面的親疏水特性。第一個系統為具有相同的PPO分子量(~2 k)，但不同的PEO/PPO的比例(~20/80, 40/60以及80/20, w/w)；第二個系統為具有不同的總分子量(~9, 11和 15 k)，但具有相同的PEO/PPO比例(80/20, w/w)。以表面電漿共振儀來即時量測共聚物於疏水表面CH3-SAM的吸附量及蛋白質吸附於共聚物表面的吸附量。本研究將系統地探討PEO-PPO-PEO分子量、PEO/PPO比例與不同的鹽離子強度對於單一蛋白(Fibrinogen和BSA)和混合蛋白吸附行為的影響。實驗發現經由PluronicTM F108所處理的表面於最佳條件下(分子量~15 k和PEO/PPO比例為80/20)具有高度的抗非特定蛋白吸附的能力。本研究中發現經由PEO-PPO-PEO共聚物所改質的表面，可以藉由控制共聚物於的表面堆疊密度來達到超低生物沾黏的表面，如透過控制共聚物之分子量、親疏水的比例和親水基團的覆蓋程度。

關鍵字

表面電將共振儀 ； 三團聯共聚物 ； 抗生物沾黏表面 ； 生物相容性

並列摘要

A well-controlled biocompatible nonfouling surface is significant for biomedical requirements, especially for the improvement of biocompatibility. We demonstrate the low or nonfouling surface by coating hydrophobic-hydrophilic triblock copolymer of poly(ethylene oxide)-poly(propylene oxide)- poly(ethylene oxide) (PEO-PPO-PEO) on the CH3-terminated self-assembled monolayer (SAM). Two types of copolymers are used to modify the surface, one with different PEO/PPO ratio (~20/80, 40/60, and 80/20, w/w) but the same ppo molecular weight (~2 k), the other with different copolymer MWs (~9, 11, and 15 k) but the same PEO/PPO ratio (80/20, w/w). In situ surface plasmon resonance (SPR) sensor is used to evaluate polymer adsorption on the SAMs and subsequent protein adsorption on the copolymer-treated surface. The effect of PEO-PPO-PEO molecular weight, PPO-to-PEO ratio, and ionic strength on protein adsorption from single protein solution of Fibrinogen, BSA, and complex mixed proteins are systematically investigated. A PluronicTM F108 treated surface is highly resistant to non-specific protein adsorption under the optimized conditions (MW of 15 k and PEO/PPO ratio of 80/20). This work demonstrates that the PEO-PPO-PEO polymer is able to achieve ultra low fouling surface modification by controlling surface packing density of polymers (molecular weight, hydrophobic/hydrophilic ratio, and hydrophilic group coverage).

並列關鍵字

biocompatibility ； nonfouling surface ； triblock copolymers ； PEO-PPO-PEO ； surface plasmon resonance

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