以常壓輝光介電質放電製備表面雙離子化 聚丙烯纖維不織布於人體血液減除白血球 的控制

Surface Zwitterionization of Polypropylene Nonwoven Fabric for the Control of Human Blood Leukodepletion by Dielectric Barrier Discharge Plasma

陳永治(Yung-Chih Chen)

指導教授 ： 魏大欽

中原大學/工學院/化學工程研究所/碩士(2020年)

https://doi.org/10.6840/cycu202000619

摘要

全血在異體運輸的過程中，白血球已被證實有導致多種不良反應的可能性，本研究以常壓輝光介電質放電之電漿系統( Line Source DBD Plasma System )接枝磺基甜菜鹼共聚物（Sulfobetaine Methacrylate, SBMA）於聚丙烯纖維不織布膜材，探討其應用於人體血液減除白血球之可行性。
研究發現，磺基甜菜鹼共聚物可快速接枝於聚丙烯膜材，改變常壓電漿掃描次數可製備不同接枝密度的改質膜材，接枝密度最大可達0.5 mg/cm2以上。XPS結果顯示，電漿改質膜材之正反面皆成功接枝了磺基甜菜鹼共聚物，然而膜材背面比正面有較多的雙離子官能基，證實了單面電漿的處理可以有效的對膜材整體改質，但電漿活性物種仍可能局部破壞接枝膜之雙離子官能基。在全血過濾方面，磺基甜菜鹼作為良好的生物相容性材料對其過濾性能提升性能有強大助益；經過 5層疊加的改質膜材可移除血液中99.7%的白血球，接近美國法規減白血品之要求(<0.01×103/μL)，且其紅血球及血小板之移除率展現出與商業膜相似性能。進一步探討，在全血貼附的測試中發現，相較於原始基材，電漿改質膜材之紅血球及白血球貼附量都可以降到10%以下，更因為改質膜材之抗生物分子沾黏特性而能有效的使血球脫附，本研究可分離出全血中的26%白血球，並且膜材所捕獲之白血球約有43%可有效釋放。

關鍵字

雙離子 ； 血球脫附 ； 抗沾黏 ； 電漿接枝

並列摘要

In blood transfusion, leukocyte has been proven to be associated with multiple possible adverse effects. In this study, line source DBD plasma system is used to graft sulfobetaine methacrylate (SBMA) on polypropylene nonwoven fibers to investigate the feasibility of reducing leukocyte from human blood.
In this study, SBMA can be grafted rapidly on polypropylene nonwoven fiber. By changing scanning number of atmospheric-pressure plasma, modified membranes with different grafting densities were successfully prepared, which has maximum grafting densities over 0.5 mg/cm2. XPS results showed that both side of the membranes were grafted by SBMA successfully. However, the back side of the membrane obtained more zwitterionic functional group than that of the front side, which proved single-sided plasma treatment can modify the entire membrane efficiently, but the plasma activated species is likely to partially damage the functional group. For whole blood filtration, as an excellent biocompatible material, SBMA is beneficial for improving filtration performance. With five layers of modified membranes, 99.7 % of leukocyte can be removed from the blood which is close to the requirement of American regulations of leukocyte reduced products (< 0.01 × 103 /μL). The removal rate of erythrocyte and thrombocyte also show the resemble performance as that of the commercial membrane. For further investigation, in whole blood attachment test, the area of attachment of erythrocyte and thrombocyte of plasma modified materials can be reduced to under 10 % compared to the original substrate. Due to its anti-fouling properties, PP-g-SBMA could effectively separates and detach 26% of leukocyte in the whole blood and 43% of leukocyte could be detached from the membrane.

並列關鍵字

zwitterionic ； desorption of leukocyte ； anti-fouling ； plasma grafting

參考文獻

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