氧化鋅奈米線指叉電極晶片進行免疫反應之研究

在這項研究中，提出一種簡單且成本低的方法能將氧化鋅奈米線選擇性生長在所需的位置，將水熱法結合微影製程技術，利用正負光阻不同特性來達到lift off(掀離)的效果，便能有效達到選擇性生長的目的。使用印刷電路板（PCB）作為基板，利用微影製程的方法將PCB板上的銅蝕刻出指叉電極圖案，將奈米線生長於指電極間隙與全面生長奈米線做比較，利用金屬親和層析法，氧化鋅奈米線層可抓取標籤有具組氨酸的蛋白質進行特異性結合，並且通過電流-電壓圖(I-V curve)的變化來達到檢測目的。實驗結果表示，氧化鋅奈米線層於廣大範圍的蛋白質濃度具有可靠的固定能力，且能夠檢測出133ng/ml~666ng/ml的抗體濃度範圍，實驗結果也表明，奈米線生長於指叉電極間隙比生長於指叉電極上更加靈敏，在試劑量為2ul時也能清楚的分辨出不同的抗體濃度，最後在利用Matlab軟體進行曲線擬合，算出不同濃度I-V曲線圖的關係式。

關鍵字

指叉電極；氧化鋅奈米線

並列摘要

A simple and inexpensive approach to selectively synthesize the ZnO nanowires at the specific area is proposed in this study. By integrating the hydrothermal method with the photolithography process, both the positive and negative resists were used to control the growth of nanowires through lift-off concept. The printed circuit board (PCB) was adopted as the substrate. On PCB, the interdigitated electrodes (IDE) array was firstly fabricated by etching the copper foil. Two types of chips were compared: global growth of ZnO nanowires on the device and patterned ZnO nanowires between IDE. Based on the principle of immobilized metal affinity chromatography (IMAC), it is found that the ZnO nanowires have specific binding affinity to capture histidine-tagged proteins. The detection of immobilized proteins is related to the variation of the current–voltage (I–V) characteristic curve. The experimental results show that ZnO nanowires have reliable immobilization capability for a wide range of protein concentrations. In addition, the protein concentrations from 133 ng/ml to 666 ng/ml can be detected. The chip with patterned ZnO nanowires between IDE is more sensitive than that with global growth of ZnO nanowires, even for small volume of protein reagent. MATLAB was also utilized to analyze the I-V curves.

並列關鍵字

ZnO nanowires ； IDE

參考文獻

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氧化鋅奈米線指叉電極晶片進行免疫反應之研究

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