噴射式大氣低溫電漿對黑色素瘤細胞之影響研究

The Effect of Melanoma Cell by Cold Atmospheric-Pressure Plasma-Jet

陳貞穎(Chen-Ying Chen)

指導教授 ： 王明誠

中原大學/工學院/生物醫學工程研究所/碩士(2014年)

https://doi.org/10.6840/cycu201400947

摘要

近年來，噴射式大氣低溫電漿應用在生物醫學的治療已成為熱門的新穎工具。特別是，它已經被證明能夠針對癌細胞組織進行治療，且能夠誘導細胞凋亡。不同的電漿參數對細胞造成的影響也不同，如造成細胞凋亡、增生以及細胞膜的損傷，低能量的電漿會使細胞增生，高能量的電漿會使細胞死亡。本研究評估噴射式大氣低溫電漿對黑色素瘤細胞的影響，包括電漿系統、電漿處理時間、電漿處理後培養時間、細胞死亡機制以及細胞移動能力，目標為在最短時間內優化電漿處理的實驗參數，進而使黑色素瘤細胞死亡，並不會對纖維母細胞造成極大的影響。該設備在32 kV 的工作電壓與126.2 kHz的工作頻率下激發電漿，此激發狀態的電流為0.22 A，消耗功率為29.9 W，氣體流量4 slpm時，電漿激發長度為24 mm，激發過程中最高溫度為31oC。利用光學放射光譜儀檢測噴射式大氣低溫電漿可於391.32、668.45、706.21 和728.16 nm觀察到氦氣的放射光譜；而在777.32、845.74 和927.28 nm 可以觀察到氧氣的光譜。本研究成功利用電漿抑制黑色素瘤細胞生長及移動能力，其參數為處理時間420秒內，並觀察到細胞以凋亡方式死亡，未來可應用於治療人類黑色素皮膚癌。

關鍵字

細胞凋亡 ； 黑色素瘤細胞 ； 噴射式大氣低溫電漿

並列摘要

In recent years, cold atmospheric-pressure plasma and their applications in biomedical treatments have become hot emerging novel tool. Especially, it has been proven to for the cancer cell, and be able to induce apoptosis. The different parameters of plasma treatment of cell cause different result, including apoptosis, proliferation and cell membrane damage. Low energy plasma is relatively cell proliferation while treatment at longer exposures led to cell death. In this study, cold atmospheric-pressure helium plasma-jet treatment on melanoma cell, include plasma system, plasma treatment time, incubation time after plasma treatment, mechanism of death and cell migration. The main goal of this study is to induce cell death in short time in melanoma cell by optimization of plasma parameters. It’s not cause significant effect on fibroblasts. The applied voltage and the discharge current of a damping waveform is respective 32 kV and 0.22 A, the frequency is 126.2 kHz, the total power of plasma-jet is 29.9 W, the best plasma length and applied temperature is respective 24 mm and 31oC at gas flow 4 slpm. Diagnose the active species in the helium plasma by optical emission spectroscopy (OES). The emission spectrum of helium can be observed at 391.32, 668.45, 706.21 and 728.16 nm; The spectrum of oxygen can be observed at 777.32, 845.74 and 927.28 nm. We successfully inhibit melanoma cell viability and cell migration by cold atmospheric-pressure plasma-jet. Finally, we get the optimization of plasma parameters, the treatment time is in 420s for cell apoptosis. In the future, cold atmospheric-pressure plasma-jet has potential in the cancer treatment applications.

並列關鍵字

Cold Atmospheric-Pressure Plasma-Jet ； Melanoma cell ； Cell Apoptosis ； HASH(0x1b79ae30)

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