以濕式誘導相分離與蒸氣誘導相分離不同的技術製備聚乙二醇酯之聚氟化乙二
烯與其動態抗沾黏能力之探討

本研究將所製備之雙親性嵌段共聚高分子PS-b-PEGMA添加於聚偏氟乙烯鑄膜液中，並使用濕式誘導相分離(LIPS)程序與蒸氣誘導相分離(VIPS)程序成形超過濾與微過濾聚偏二氟乙烯高分子薄膜，並系統性探討其抗生物沾黏特性。第一部分，使用LIPS與VIPS程序所製備之薄膜將以傅里葉轉換紅外光譜、X-射線光電子光譜、電子顯微鏡、原子力顯微鏡進行鑒定與比較。接著先評估以LIPS程序所製備之超過濾薄膜的血液相容性，來確認其是否可適用於血液接觸之相關應用。所製備之薄膜在單一成分之蛋白質溶液(含纖維蛋白原、免疫血清球蛋白、人血清白蛋白)與寡含血小板血漿溶液中，透過酵素免疫分析法來量測其蛋白質吸附量，並以電子顯微鏡與鐳射掃瞄共軛聚焦顯微鏡來觀察各式血球(血小板、紅血球與白血球)於膜面之貼附情形。此外，溶血實驗與凝血時間也被分析與觀察。最後評估以VIPS程序所製備之微過濾薄膜於微藻培養回收之應用，除了同時測試薄膜系統的靜態抗蛋白吸附能力外，在動態條件下的防污能力也是本研究的主要挑戰的討論焦點。結果顯示，可使用LIPS程序成功製備出PEGylated PVDF薄膜系統，在較高的PS-b-PEGMA添加含量可有效改善其血液相容性質，特別是優越於未改質之PVDF薄膜的抗凝血性質。另一方面，以VIPS程序製備之PS-b-PEGMA-4薄膜顯示非常高的通透量與超過97.7%的微藻截留率，指出其具高效率之微藻培養回收能力。最重要的是，以LIPS與VIPS製程所製備之薄膜系統皆呈現優秀的抗污能力，其不可逆污染性質被明顯最小化，且透過去離子水即可簡單將薄膜進行物理清洗來恢復其通量損失。因此，本研究所製備之PS-b-PEGMA/PVDF薄膜系統同時具有抗污、血液相容性與高液體通透量，可廣泛地提供具有潛力的特定應用，如血液透析和微藻回收。

關鍵字

PS-b-PEGMA ； PVDF ；微藻培養；抗污；血液相容性

並列摘要

The present work deals with the preparation of ultrafiltration (UF) and microfiltration (MF) membranes with enhanced fouling resistant ability by using two different methods, liquid- induced phase separation (LIPS) and vapor-induced phase separation (VIPS), after in-situ modification of the PVDF solution using amphiphilic PS-b-PEGMA block copolymers as additive. In the first section, using FT-IR, XPS, SEM, AFM, and contact angle meter, the surface chemistry, morphology, and hydrophilic properties of LIPS along with VIPS membranes were characterized and compared. The next part dealt with the evaluation of the hemocompatibility of UF-LIPS membranes as it was intended for blood-contacting uses. The so called ELISA test was used to assess protein adsorption from single protein solution (FN, γ-globulin, HSA) and PPP solution. Also, blood cells (thrombocytes, erythrocytes, and leukocytes) attachment was investigated by means of SEM and confocal microscopy. Additionally, hemolysis experiments and measurements of plasma-clotting time were carried out. The final portion was dedicated to the assessment of MF-VIPS membranes’ efficiency when applied in microalgae harvesting. Apart from testing the static protein resistance of both membrane systems, the antifouling ability in dynamic conditions which is the primary focus of this study were also challenged. Results demonstrate that PVDF membranes were successfully prepared with a facile approach. PEGylated membranes prepared by LIPS process containing the highest copolymer content showed improved blood compatibility; particularly the anticoagulant property was evidently enhanced compared to the virgin PVDF membranes. On the other hand, PS-b-PEGMA-4 VIPS membranes, exhibited exceptionally high permeate flux and rejection ratio of more than 97.7% that suggests efficient harvesting of microalgae. Most importantly, both LIPS and VIPS membranes exhibited outstanding antifouling capability as irreversible fouling was notably minimized and simple physical cleaning by DI water was able to recover flux loss. Therefore, PS-b-PEGMA/PVDF membranes with integrated antifouling property, blood compatibility, high permeate flux provided wide variety of specific applications for instance hemodialysis and microalgae recovery.

並列關鍵字

PS-b-PEGMA ； PVDF ； antifouling ； hemocompatibility ； microalgae

參考文獻

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[2] Şenay, R. H., Gökalp, S. M., Türker, E., Feyzioğlu, E., Aslan, A., & Akgöl, S. (2015). A new morphological approach for removing acid dye from leather waste water: Preparation and characterization of metal-chelated spherical particulated membranes (SPMs). Journal of Environmental Management, 151, 295-302.

[3] Yao, L., Zhang, L., Zhang, Y., Wang, R., Wongchitphimon, S., & Dong, Z. (2015). Self-assembly of rare-earth Anderson polyoxometalates on the surface of imide polymeric hollow fiber membranes potentially for organic pollutant degradation. Separation and Purification Technology, 151, 155-164.

[4] Chen, W., Su, Y., Peng, J., Zhao, X., Jiang, Z., Dong, Y., . . . Liu, J. (2011). Efficient wastewater treatment by membranes through constructing tunable antifouling membrane surfaces. Environmental Science and Technology, 45(15), 6545-6552.

[5] Zhu, W.-P., Gao, J., Sun, S.-P., Zhang, S., & Chung, T.-S. (2015). Poly(amidoamine) dendrimer (PAMAM) grafted on thin film composite (TFC) nanofiltration (NF) hollow fiber membranes for heavy metal removal. Journal of Membrane Science, 487, 117-126.

國際替代計量

以濕式誘導相分離與蒸氣誘導相分離不同的技術製備聚乙二醇酯之聚氟化乙二烯與其動態抗沾黏能力之探討

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