In this thesis, the ISFET with the Ω-shape nano-wire channels were fabricated with the nano imprint technology. The characterizations of the ISFET with APTES/oxide stacked sensing membrane were studies. In our results, the ISFET with the Ω-shape nano-wire channels were fabricated successfully. The Ω-shape nano-wire channels with the top width of about 110~220 nm and the bottom width of about 70~170 nm were fabricated. The sensitivity and hysteresis of ISFET with the Ω-shape nano-wire channels were all improved. This technology will be suitable for the future bio-sensing applications.