薄層分離槽的一些初步探討

本篇論文透過相關文獻資料的查詢，得知薄層分離槽可以減少實驗中所需要的試劑用量，甚至能根據實驗者所需，設計出多段式的通道裝置，將多個實驗步驟濃縮在一個分離槽裝置上。故初步嘗試製作出薄層分離槽裝置，並透過實驗去比較使用不同的組件材質和組合方法之優缺點，本實驗發現一些容易導致製作失敗的原因，並加以記錄、修正、避免，最終得出較為合適的簡易薄層分離槽裝置製作方法。後續也透過磁場分離的相關技術，去檢視分離槽是否製作成功，以及是否有要修正的地方。首先將薄層分離槽的中央對齊在強力磁鐵上，並以膠帶固定，再將配製好的磁性奈米顆粒溶液注入分離槽中，使奈米顆粒在分離槽中與雜質進行分離後，沉積在特定位置。由於本實驗中採用的是直徑低於100 nm的氧化鐵奈米顆粒，若在實驗過程中有雜質參入，會顯著的污染樣品和影響實驗結果，故經過光學顯微鏡初步觀察奈米顆粒的分布狀況後，也更進一步利用原子力顯微鏡進行觀測，確認分離和沉積的效果是否符合預期。

關鍵字

薄層分離槽；原子力顯微鏡

並列摘要

By the literature data search, it is known that the thin separation cells method can reduce the volume of sample required in the experiment as well as can design multi-stage channel to perform multiple experiments in a single cell. This preliminary research discover some of experimental methods to get suitable and simple thin separation cells. Also discussed the advantages and disadvantages of the thin-layer separation method by comparing different aspects. Here we also applied the magnetic field separation technique by using <100 nm iron oxide nanoparticles to check the performance of constructing thin separation cells. In detail, the suitable concentrated solution of nanoparticles was injected into the channel which is center fixed on the external magnet. Hence the pure iron oxide particles were deposited in the channel path and impurities were drained out. Further distribution of nanoparticles was initially observed through an optical microscope than an atomic force microscope to confirm the effect of separation and deposition meets expectations.