氧化鐵磁性顆粒修飾網版印刷碳電極對嘉磷塞之電化學分析

Electrochemical Determination of Glyphosate based on Magnetic Iron Oxide Nanoparticle-Modified Screen-Printed Carbon Electrodes

謝文武(Man-Mo Tse)

指導教授 ： 鄭淑華

國立暨南國際大學/科技學院/應用化學學系/碩士(2020年)

https://doi.org/10.6837/ncnu202000316

摘要

嘉磷塞是一種有機磷除草劑，廣泛使用在農業，研究指出嘉磷塞可能有致癌風險，為了避免過量使用，衛生福利部食品藥物管理署規定於不同的食品中嘉磷塞的殘留容許量在0.1-10 ppm之間。由於嘉磷塞沒有電化學活性，因此，電化學分析嘉磷塞存在一定難度，我們希望可以開發出簡單、快速的嘉磷塞電化學偵測方法。
本研究以簡單的共沉澱法製備出磁性氧化鐵奈米顆粒(Fe3O4)，並成功將其修飾在網版印刷碳電極 (screen-printed carbon electrode, SPCE)上，即氧化鐵修飾電極(SPCE/Fe3O4)，並將修飾電極浸泡在鐵氰化鉀(K3[Fe(CN)6])與嘉磷塞混合溶液中，再透過循環伏安法 (Cyclic voltammetry, CV)以及安培法(Amperometry, i-t)，在修飾電極表面上電化學反應生成普魯士藍，藉由普魯士藍氧化還原訊號的大小，間接檢測嘉磷塞。我們利用XPS、FT-IR、TEM以及SEM對氧化鐵奈米顆粒及其修飾電極進行鑑定，結果顯示氧化鐵奈米顆粒平均粒徑約為15 nm左右，並成功以上述方法合成普魯士藍。此方法的靈敏度為 0.1454 A/M，線性範圍介於 50 ~ 1000 M，偵測極限為1.38 M (S/N=3)，相當於0.233 ppm。

關鍵字

網版印刷碳電極 ； 氧化鐵 ； 鐵氰化鉀 ； 普魯士藍 ； 嘉磷塞

並列摘要

Glyphosate is an organophosphorus compound, which is widely used in agricultural. To avoid the abuse of glyphosate, the Food and Drug Administration of the Ministry stipulates that the maximum residue levels of glyphosate in food are limited in between 0.1 to 10 ppm. Glyphosate has no electrochemical activity and in this work, we hoped to develop a simple and rapid electrochemical method for glyphosate determination.
In this study, magnetic iron oxide nanoparticles (Fe3O4) were prepared by a simple co-precipitation method, and then successfully modified on screen-printed carbon electrode (SPCE), namely iron oxide modified electrode (SPCE/Fe3O4). The as-prepared modified electrode is immersed in a mixed solution containing potassium ferricyanide (K3[Fe(CN)6]) and glyphosate. Electrochemical generation of Prussian blue (PB) film on the electrode surface was observed by reduction potential treatment. The redox signal of Prussian blue is related to the concentration of glyphosate in solution. The iron oxide nanoparticles and its modified electrode were characterized using XPS、FT-IR、TEM and SEM. The results show that the average particle size of the iron oxide nanoparticles is about 15 nm, and the Prussian blue is successfully synthesized by the above method. The method sensitivity was 0.1454 A/M. A linear response to glyphosate in the range of 50 ~ 1000 M was observed. The detection limit was 1.38M (S/N=3), equal to 0.233 ppm.

並列關鍵字

screen-printed carbon electrode ； iron oxide ； potassium ferricyanide ； Prussian blue ； glyphosate

參考文獻

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