以低溫共沉積氧化鋅奈米粒與抗癌藥物於紙基板對癌症細胞毒性之研究

Cytotoxicity against Cancer Cells of ZnO Paper Co-deposited with Anti-Cancer Drugs at Low Temperature

李岳龍(Yueh-Lung Li)

指導教授 ： 吳幼麟

國立暨南國際大學/科技學院/電機工程學系/碩士(2016年)

摘要

氧化鋅(ZnO)奈米粒對人體健康有害，但是也被證明對某些癌症細胞具有毒性而對某些人類正常細胞卻是沒有影響。本論文主要探討以低溫的方式使用化學沉積法，讓氧化鋅奈米粒及抗癌藥物共沉積在纖維紙上，並以此沉積有氧化鋅奈米粒及抗癌藥物之纖維紙為測試工具，進行癌細胞的耐受性測試，透過MTT assay(3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay)分析來觀察癌細胞在紙上的存活率。論文中我們將探討不同溫度下的製程方式與不同沉積時間對於癌細胞毒性的變化。

確認氧化鋅奈米粒是否有沉積在纖維紙基板上，我們藉由X-射線繞射分析(XRD)分析纖維紙基板上的氧化鋅奈米粒的結晶結構，並以傅立葉轉換紅外線光譜儀(Fourier Transform Infrared Spectrometer，FTIR)分析抗癌藥物在紙基板與氧化鋅共沉積的分子結構是否會因為共沉積的時間或是溫度的不同產生變化。 最後將癌細胞培養於氧化鋅紙基板上，透過MTT assay分析並利用酵素結合免疫吸附法(enzyme-linked immunosobent assay，ELISA)檢測細胞存活率。的結構有所不同，可判斷使用本實驗的化學沉積法可以讓抗癌藥物成功地與氧化鋅共沉積在紙基板上，沉積時間的長度與實驗製成的溫度均會影響藥物的分子結構，進而影響對於癌細胞的毒性。在60℃的製程下，抗癌藥的化學鍵將會被破壞，無法沉積在纖維紙上。在相同的沉積時間下，高溫製程的在48小時的沉積時間毒性會達到最大，低溫製程則要到72小時。在相同極短沉積時間下，加入Staurosporine的低溫製程共沉積紙毒性最佳；加入Staurosporine的低溫製程對細胞毒性，比其他低溫製程毒性來的好。

關鍵字

化學沉積法 ； 氧化鋅奈米粒 ； 細胞活性檢測 ； 共沉積 ； 細胞毒性

並列摘要

Zinc oxide (ZnO) nanoparticle is harmful for human health, while it has been found to exhibit cytotoxicity on several human glioma cell lines but exerting no effect on normal human astrocytes. The purpose of this research aims at investigating the feasibility of using ZnO paper prepared at low temperature with co-deposition of anti-cancer drugs for cytotoxicity effect against lung adenocarcinoma cancer cell lines A549 and H1299. Chemical reduction of zinc acetate dehydrate at 25°C and 60°C, respectively, was used to deposit ZnO nanoparticles onto the cellulose paper substrate. Some of the ZnO papers were co-deposited with anti-cancer drugs – Iressa and Staurosporine. These papers were then used as a testing tool for the cytotoxicity of ZnO nanoparticles via the MTT (3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay analysis.

X-ray diffraction (XRD) was used to determine the crystallinity of ZnO nanoparticles deposited on the cellulose paper and Fourier Transform Infrared Spectroscopy (FTIR) to confirm the chemical structure of anti-cancer drugs co-deposited on the paper substrate. We used the MTT (3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay combined with enzyme-linked immunosorbent assay analysis to check the cell viability. Our experimental results confirm that ZnO nanoparticles co-deposited with anti-cancer drugs on cellulose paper can be used as a testing platform for cytotoxicity test.

並列關鍵字

Chemical reduction ； ZnO nanoparticle ； MTT assay ； Co-deposited ； cytotoxicity of Zinc oxide

參考文獻

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