對-胺基苯磺酸修飾網版印刷碳電極的電化學研究

Electrochemistry of the 4-aminobenzene sulfonate-modified screen-printed carbon electrodes

曾華勇(Hua-Yung Tseng)

指導教授 ： 鄭淑華

國立暨南國際大學/科技學院/應用化學系生物醫學碩士班/碩士(2013年)

https://doi.org/10.6837/NCNU.2013.00213

摘要

多巴胺（dopamine, DA）在人體中是一個重要的神經傳導物質。一般而言，電化學偵測多巴胺時存有的許多干擾化合物，如抗壞血酸（ascorbic acid, AA）和尿酸（uric acid, UA）的一個主要問題。為了解決此問題，因此使用各種材料去修飾電極以達到去除干擾或是將訊號分離的效果。
在這項研究中，將4 –胺基苯磺酸（sodium 4-aminobenzenesulfonate, 4-ABSA）使用電化學方法以（A）胺類自由基（B）重氮鹽陽離子兩種方式，修飾網版印刷碳電極（SPCE）。利用 SPCE / 4-ABSA，在pH 7.0 的緩衝溶液中分別偵測AA，DA和UA。而 AA，DA和UA的氧化電位分別為0.43，0.29和0.54 V。利用SPCE / Ph-SO3在pH 7.0 的緩衝溶液中偵測AA，DA和UA。而AA，DA和UA的氧化電位分別為 0.59，0.37和0.53 V。但是當偵測混合溶液 時，皆只有DA 和UA混合溶液氧化峰可以分離。
利用SPCE / 4-ABSA偵測在含有固定濃度100 μM DA與不同濃度UA中，當UA濃度偵測範圍在10 ~ 100 μM，線性關係為I＝0.0333*[UA]－0.2992。而用SPCE / 4-ABSA偵測在含有固定濃度100 μM UA與不同濃度DA中，當DA濃度偵測範圍在1 ~ 200 μM，線性關係為I＝0.08532*[DA]＋0.7402。

關鍵字

多巴胺 ； 4 –胺基苯磺酸 ； 胺類自由基 ； 重氮鹽陽離子 ； 壞血酸 ； 尿酸

並列摘要

Dopamine (DA) is an important chemical neurotransmitter in the human body. Electroanalysis of dopamine has been studied in the literatures. A major problem of electrochemical detection of DA is the coexistence of many interfering compounds, such as ascorbic acid (AA) and uric acid (UA). In order to solve this problem, various materials were used to modify the electrode surface, and then improve the analytical performances.
In this study, the sodium 4-aminobenzene sulfonate was used to modify screen-printed carbon electrode by two electrochemical methods. Detecting individual AA, DA and UA in the pH 7.0 buffer solution was achieved by SPCE / 4-ABSA; the oxidation potential of AA, DA and UA were at 0.43, 0.29 and 0.54 V, respectively. Detecting individual AA, DA and UA in the pH 7.0 buffer solution was achieved by SPCE / Ph-SO3; the oxidation potential of AA, DA and UA were at 0.59, 0.37 and 0.53 V, respectively. In the mixed solution, only the peaks of DA and UA can be separated.
In the presence of 100 μM DA, the working range for UA was 10 ~ 100 μM using SPCE / 4-ABSA, and the linear relationship was I＝0.0333*[UA]－0.2992. In a fixed concentration of 100 uM UA, the working range for DA was 1 ~ 200 μM using SPCE / 4-ABSA, and the linear relationship was I = 0.08532 * [DA] +0.7402.

並列關鍵字

dopamine ； 4-aminobenzene sulfonate ； amine radical ； diazonium salt cationic ； ascorbic acid ； uric acid

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