奈米金的表面電漿共振特性及DNA感測之研究

近來，奈米金屬結構的產生對近場光學的研究有相當深廣的幫助。奈米金屬結構也常應用在奈米醫學領域上。在本研究中，我們以二種方式去製作出奈米金屬結構，其中有運用到奈米球自組裝微影術 (Nanosphere lithography; NSL)、化學還原奈米金等方式。我們以這兩種製程方法製作出奈米金屬結構，再去觀察奈米金屬結構的吸收光譜圖譜去了解其局部表面電漿共振現象 (Localize Surface Plasmon Resonance, LSPR)。由於LSPR對折射率的變化相當敏感，我們進一步證實了我們製作的奈米金屬結構可以用來作為DNA感測器。奈米球自組裝微影術必須使用自組裝技術來生成一單層奈米級聚苯乙烯(Polystyrene, PS)奈米球薄膜光罩來製造出一奈米金屬結構。然而，由於受限於實驗設備，我們無法成功地製作奈米金屬結構。在經過長時努力之後，本實驗改變了製程，使用化學還原的方式來製造出粒徑10~15nm左右的奈米金粒子溶液，在實驗中，我們比較了奈米金溶液LSPR特性在水相與有機相的差異性。同時，我們嘗試將奈米金粒子自組裝在玻璃基板上，預期做出一微流道元件能用來偵測DNA。實驗結果表示，我們成功地做出了一能偵測波長位移DNA信號在濃度100nM的DNA感測器。

關鍵字

表面電漿共振；奈米金屬；自組裝微影

並列摘要

Recently, the nano-metal structures that can produce some interested near-optical properties have been widely and deeply studied. In our work, we used two methods , nanosphere lithography and chemical reduction, to fabricate nano-metal structure ans study their localize surface plasmon resonance (LSPR) by characterizing the absorption spectra. Since the LSPR is sensitive to the refractive index modulation, we further proved our fabricated nano-metal structure could be used as a DNA sensor. Nanosphere lithography has to use a self-assembly technique to generate a mono-layered and nano-sized polystyrene (PS) thin film which serves as a mask for fabricating the nano-metal structure. However, due to the limitation of etching equipment we were unable to successfully fabricate the nano-metal structure. After a long effort, we changed the fabrication procedure by using a chemical reducation method and a particle size of about 10 ~ 15nm nanometer gold particle solution was successfully fabricated. In the experiment, we compared the optical properties of LSPR in the nano-gold solution which could be fabricated either in the water phase or the organic phase. The gold nanoparticles were self-assembled in the glass substrate which was expected to make a microfluidic channel device for DNA sensing. The resurts indicated a successful DNA sensor which has a detectable wavelength shift of DNA signal from 100 nM target DNA.