原子力顯微鏡對細胞表面特性之探討

Study the surface properties of cells using atomic force microscopy

蔡欣玫(Hsin-Mei Tsai)

指導教授 ： 傅傳博

國立暨南國際大學/科技學院/應用化學系/碩士(2010年)

摘要

原子力顯微鏡的優點是生物樣品可以在其生理溶液下測量，並且保持最原始的特性，也提供了高解析度生物結構的三維影像圖。
在這篇論文中，使用原子力顯微鏡在生理環境下觀察細胞的表面形貌及機械性質。未修飾與修飾原子力顯微鏡探針應用於活體細胞做力曲線特性分析。此外，在奈米尺度下細胞的機械性質依據赫茲模型(Hertz model)計算出楊氏系數(Young’s modulus)，做數據的定量分析。

關鍵字

原子力顯微鏡

並列摘要

The advantage of AFM is that biology samples can be kept origin properties which measure under physiological solutions; it also provides three-dimensional (3D) images of biological structures with high resolution.
In this thesis, the surface morphologies and the mechanical properties of the cells were measured by using AFM under physiological environments. The unmodified/ modified AFM tips were applied to characterize the force curve of the living cells. In addition, the Young’s modulus of the cell was deduced by Hertz model.

並列關鍵字

Atomic force microscopy

參考文獻

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